社区和医院获得性耐甲氧西林金黄色葡萄球菌MLST联合SCCmec基因分型及耐药性比较

发布时间：2018-02-26 01:16

本文关键词： 社区获得性医院获得性金黄色葡萄球菌多位点序列分型葡萄球菌mec盒式染色体　出处：《中国抗生素杂志》2017年01期 　论文类型：期刊论文

【摘要】：目的了解社区获得性耐甲氧西林金黄色葡萄球菌(CA-MRSA)和医院获得性耐甲氧西林金黄色葡萄球菌(HAMRSA)分离株的耐药性及遗传背景。方法对CA-MRSA和HA-MRSA进行多位点序列分型(MLST)检测,应用多重聚合酶链式反应(PCR)技术对CA-MRSA和HA-MRSA进行SCCmec基因分型;采用Walk WAY96PLUS PC33药敏板联合K-B纸片扩散法检测CA-MRSA和HA-MRSA抗菌药物敏感性。结果 63株HA-MRSA共检测出2个基因型,4株CA-MRSA共检测出4个基因型,二者遗传背景完全不同。HA-MRSA对红霉素、克林霉素、四环素、庆大霉素、左氧氟沙星和环丙沙星耐药率显著高于CAMRSA(均P0.05)。结论滨州医学院附属医院CA-MRSA和HA-MRSA分型及主要流行株存在差异,CA-MSSA药物敏感性明显高于HA-MRSA;MLST联合SCCmec基因分型方法是MRSA分子流行病学分析的有效方法。
[Abstract]:Objective to investigate the drug resistance and genetic background of community acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) and hospital acquired methicillin-resistant Staphylococcus aureus (HAMA) isolates. Methods CA-MRSA and HA-MRSA were detected by multilocus sequence typing. Multiplex polymerase chain reaction (PCR) technique was used to detect the SCCmec genotyping of CA-MRSA and HA-MRSA. Walk WAY96PLUS PC33 plate combined with K-B disk diffusion method was used to detect the antimicrobial susceptibility of CA-MRSA and HA-MRSA. Results A total of 2 genotypes and 4 CA-MRSA genotypes were detected in 63 HA-MRSA strains. The genetic background of the two genotypes was completely different from that of Erythromycin. Clindamycin, tetracycline, gentamicin, The drug resistance rates of levofloxacin and ciprofloxacin were significantly higher than those of CAMRSA.Conclusion there are differences between CA-MRSA and HA-MRSA typing and major epidemic strains in the affiliated Hospital of Binzhou Medical College. CA-MSSA drug sensitivity is significantly higher than that of HA-MRSA-MLST combined with SCCmec genotyping method. The results indicate that the drug sensitivity of CA-MSSA is higher than that of HA-MRSA-MLST combined SCCmec genotyping. An effective method for epidemiological analysis.
【作者单位】：滨州医学院附属医院检验科;滨州医学院附属医院感染管理办公室;滨州医学院病原生物学教研室;
【基金】：滨州市科技发展计划项目(No.2013ZC1711,No.2013ZC1802) 山东省高等学校科技计划资助项目(No.J13LK16) 滨州医学院科技计划(No.BY BY2013KJ02)
【分类号】：R440

【相似文献】