马尔尼菲蓝状菌氟康唑耐药基因的表达

发布时间：2018-02-26 01:09

本文关键词： 马尔尼菲蓝状菌马尔尼菲青霉病氟康唑耐药基因耐药性诱导耐药实时荧光定量PCR　出处：《中国皮肤性病学杂志》2017年06期 　论文类型：期刊论文

【摘要】：目的比较马尔尼菲蓝状菌(Talaromyces marneffei,T.marneffei)耐药株和敏感株氟康唑(Fluconazole,FCZ)耐药基因在转录水平上表达量的差异,为马尔尼菲青霉病(Penicilliosis marneffei,PSM)临床用药及预后监测提供参考依据。方法采用FCZ浓度递增法对11株酵母相T.marneffei进行体外诱导耐药,按临床实验室标准委员会(NCCLS)颁布的微量稀释法测其最小抑菌浓度(minimal inhibitory concentration,MIC值),通过实时荧光定量逆转录-聚合酶链反应(Real-time fluomgenetic quantitative PCR,FQ-PCR)测定耐药株及敏感株FCZ耐药基因的表达量,比较分析T.marneffei的MIC值变化前后与FCZ耐药基因表达量的关系。结果 11株酵母相T.marneffei经诱导后,8株T.marneffei达到耐药判断标准,FCZ耐药基因在T.marneffei中均表达,表达量由诱导前的2.56±1.84上升至7.50±3.00,差异有统计学意义(P㩳0.05);每株T.marneffei经FCZ诱导后FCZ耐药基因表达量均有不同程度的上升,上升幅度范围在1.8~16.4倍之间。结论采用FCZ浓度递增法可成功在体外诱导酵母相T.marneffei耐药,T.marneffei对FCZ的耐药性可能与FCZ耐药基因的表达有关。
[Abstract]:Objective to compare the transcriptional expression levels of fluconazole-resistant and susceptible fluconazole-resistant genes in Talaromyces marneffeii (T. marneffeii) strains. To provide reference for clinical use and prognosis monitoring of Penicilliosis marneffeii PSM.Methods FCZ concentration increasing method was used to induce resistance of 11 strains of yeast T. marneffei in vitro. Minimal inhibitory concentration and minimal inhibitory concentration were measured by microdilution method promulgated by the Committee of Clinical Laboratory Standards. Real-time fluomgenetic quantitative PCRFQ-PCRR was used to determine the expression of FCZ resistance genes in drug-resistant and sensitive strains by real-time quantitative reverse transcription-polymerase chain reaction (real-time fluomgenetic quantitative PCRQ-PCRR). Results 11 strains of T. marneffei were induced by T. marneffei and 8 strains of T. marneffei met the criteria of drug resistance and the FCZ resistance genes were all expressed in T. marneffei. The expression level increased from 2.56 卤1.84 before induction to 7.50 卤3.00 (P < 0.05). After induced by FCZ, the expression of FCZ resistance genes in each strain of T. marneffei increased in varying degrees. Conclusion the resistance of T. marneffei to FCZ in yeast phase can be induced successfully by increasing the concentration of FCZ in vitro, which may be related to the expression of FCZ resistance gene.
【作者单位】：广西右江民族医学院附属医院;广西医科大学;广西医科大学第一附属医院;
【分类号】：R519

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