RASSF1A与GSTP1基因甲基化检测对前列腺癌诊断价值的研究

发布时间：2018-02-26 05:16

本文关键词： 基因甲基化前列腺癌诊断　出处：《中华肿瘤防治杂志》2017年17期 　论文类型：期刊论文

【摘要】：目的前列腺癌的早期诊断缺乏特异性,联合基因甲基化检测可能具有一定的价值。本研究探讨RASSF1A与GSTP1基因甲基化联合检测对前列腺癌的诊断价值。方法收集滨州医学院烟台附属医院泌尿外科行手术治疗并经过病理组织学确诊的前列腺癌患者42例作为病例组,另选取同期健康体检者40名作为对照组。于术前或体检时获取血液标本,提取DNA后通过实时荧光定量甲基化特异PCR(MSP)法检测RASSF1A与GSTP1基因甲基化率,分析年龄、性别和临床分期等指标对甲基化的影响。探讨两类基因甲基化联合检测对前列腺癌诊断的敏感性和特异性。结果病例组RASSF1A基因甲基化率为66.67%(28/42),GSTP1基因甲基化率为61.90%(26/42);对照组RASSF1A基因甲基化率为15.00%(6/40),GSTP1基因为10.00%(4/40)。各临床病理特征与患者RASSF1A和GSTP1基因甲基化均不存在明显的相关性,均P值0.05。RASSF1A和GSTP1基因甲基化均为前列腺癌发病的独立危险因素,均P值0.05。联合检测RASSF1A和GSTP1基因甲基化能够明显提高诊断的敏感性(83.33%)及特异性(83.87%)。结论 RASSF1A和GSTP1基因甲基化联合检测可显著提升前列腺癌的诊断水平。
[Abstract]:Objective the early diagnosis of prostate cancer lacks specificity. The combined detection of RASSF1A and GSTP1 gene methylation may have some value in the diagnosis of prostate cancer. Methods the surgical treatment of urology in Yantai affiliated Hospital of Binzhou Medical College was collected. Forty-two prostate cancer patients treated and pathologically confirmed as the case group, In addition, 40 healthy persons were selected as control group. Blood samples were obtained before or during physical examination. The methylation rates of RASSF1A and GSTP1 genes were detected by real-time fluorescence quantitative methylation specific PCR- MSPassay, and the age was analyzed. The effect of sex and clinical stage on methylation. To explore the sensitivity and specificity of two types of gene methylation combined detection in the diagnosis of prostate cancer. Results the methylation rate of RASSF1A gene in the case group was 66.677,28 / 42%, and the methylation rate of GSTP1 gene was 61.90% / 42%. The methylation rate of RASSF1A gene in the radiation group was 15.00%. The methylation rate of GSTP1 gene was 10.00%. There was no significant correlation between the clinicopathological features and the methylation of RASSF1A and GSTP1 genes. The mean P value was 0.05. RASSF1A and GSTP1 gene methylation were independent risk factors for prostate cancer. The combined detection of RASSF1A and GSTP1 gene methylation can significantly improve the diagnostic sensitivity and specificity of RASSF1A and GSTP1 gene methylation. Conclusion the combined detection of RASSF1A and GSTP1 gene methylation can significantly improve the diagnostic level of prostate cancer.
【作者单位】：滨州医学院烟台附属医院急诊科;滨州医学院烟台附属医院病理科;
【分类号】：R737.25

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