产气荚膜梭菌NetB毒素基因重组植物乳酸杆菌疫苗免疫保护性研究

发布时间：2018-02-26 15:16

本文关键词： 产气荚膜梭菌 NetB毒素植物乳酸杆菌免疫保护　出处：《中国家禽》2017年14期 　论文类型：期刊论文

【摘要】：为了评估表达产气荚膜梭菌NetB毒素的重组植物乳酸杆菌口服活菌疫苗对鸡的免疫保护效果,分别进行了重组活菌疫苗的免疫和产气荚膜梭菌攻菌试验。免疫组雏鸡分为3组,于7~28日龄分别连续免疫p SIP409空载体植物乳酸杆菌、p SIP409-NetB重组植物乳酸杆菌和灭菌PBS;攻菌组雏鸡免疫方法同上,于43~47日龄连续口服产气荚膜梭菌(设置PBS免疫对照组和不攻菌只口服等量PBS对照组)。通过采用ELISA检测抗体动态变化,观察雏鸡体重变化和免疫器官发育情况以评价疫苗的免疫效果。结果显示,NetB组特异性抗体Ig G和s Ig A水平随日龄增长呈现上升趋势,且与未免疫对照组存在显著或极显著差异(P0.05或P0.01);NetB攻菌组各项指标在短暂下降后很快升高,且明显高于未免疫对照组雏鸡(P0.01)。NetB组周增重14日龄后均高于其他组,且35日龄开始与其他组差异极显著(P0.01);攻菌组体重变化总趋势是NetB攻菌组最高,其次是空载体攻菌组(P0.05),PBS攻菌组最低(P0.01)。免疫组在免疫结束时胸腺和脾脏指数呈明显上升趋势,NetB组的这两项指数分别在56日龄和49日龄达到最高,法氏囊指数在28日龄下降,随后稳步上升;虽然攻菌后各免疫器官指数上升趋势变缓,但NetB攻菌组的各项指数在各时间点均与其他组差异极显著(P0.01)。表明重组口服活菌疫苗对产气荚膜梭菌攻击雏鸡起到很好的保护作用,所构建的重组疫苗安全有效,结果为重组植物乳酸杆菌表达系统应用于口服疫苗提供了理论依据。
[Abstract]:In order to evaluate the immune protection effect of recombinant live Lactobacillus plantarum vaccine expressing Clostridium perfringens NetB toxin on chickens, the immunization test of recombinant live bacillus vaccine and Clostridium perfringens attack test were carried out respectively. The chicks were divided into three groups. The recombinant and sterilized Lactobacillus plantarum and sterilized Lactobacillus plantarum were continuously immunized with Lactobacillus spp on 728 days of age, respectively, and the inoculation methods of infected chicks were the same as those of inoculated chicks. Clostridium perfringens were taken orally continuously at 43 ~ 47 days of age (PBS immunized control group and non-invasive PBS control group were given orally only). The dynamic changes of antibody were detected by ELISA. The changes of body weight and the development of immune organs were observed to evaluate the immune effect of the vaccine. The results showed that the levels of specific antibodies Ig G and sig A in NetB group showed an increasing trend with the increase of age. And there was significant or extremely significant difference between the control group and the unimmunized control group. The indexes of the control group were increased rapidly after a brief decrease, and were significantly higher than that of the unimmunized control group after 14 days of week weight gain, which was higher than that of the other groups. At 35 days of age, there was a significant difference between the two groups (P 0.01), and the total trend of body weight change in the attack group was the highest in the NetB attack group. The second was the lowest P0.01C in the P0.05PBS attack group. The thymus and spleen index of the immunization group increased significantly at the end of immunization. The index of thymus and spleen reached the highest in the NetB group at the age of 56 days and 49 days, and the bursa of Fabricius index decreased at 28 days old, respectively, in the immunization group, the index of thymus and spleen increased significantly at the end of immunization, and the index of bursa of Fabricius decreased at 28 days of age. Then it rose steadily; although the increase in the index of the immune organs slowed down after the attack, However, the indexes of NetB attack group were significantly different from those of other groups at each time point, which indicated that the recombinant live oral vaccine had a good protective effect against Clostridium perfringens attack on chicks, and the recombinant vaccine was safe and effective. The results provided a theoretical basis for the application of recombinant Lactobacillus plantarum expression system to oral vaccine.
【作者单位】：黑龙江省兽医科学研究所;东北农业大学动物医学学院;大庆市畜牧兽医局;
【分类号】：S858.31

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