哈萨克羊和藏绵羊尾部脂肪组织差异表达基因和可变剪接的鉴定与分析

发布时间：2018-02-27 18:27

本文关键词： 肥脂尾差异表达基因哈萨克羊藏绵羊转录组　出处：《西北农业学报》2017年03期 　论文类型：期刊论文

【摘要】：为鉴定哈萨克羊和藏绵羊尾部脂肪组织的差异表达基因和可变剪接,分别提取6只(3公,3母)无亲缘关系的2岁成年哈萨克羊与藏绵羊尾部脂肪组织总RNA,构建2个高通量测序文库,利用TopHat2软件将短序列定位到绵羊参考基因组,采用cuffdiff软件分析基因表达丰度和差异表达基因。结果表明,共有19个基因在2个品种绵羊尾脂中的表达丰度均高于1 000FPKM,包括ADIPOQ、SPARC、VIM等。其中,2个品种尾部脂肪组织中表达量最高的转录本均定位于线粒体基因组5 331~14 154bp,该区域主要包含COX1、COX2、ATP8等基因,其表达丰度在哈萨克羊和藏绵羊中分别高达29 724.20和28 818.80FPKM。而FABP4则是表达丰度最高的核基因,在哈萨克羊和藏绵羊中的表达量分别为3 194.03和2 667.09。差异表达基因分析共鉴定出627个差异表达的基因,与藏绵羊尾部脂肪组织的表达量相比,哈萨克羊的尾部脂肪组织中共有221个上调基因和406个下调基因。其中,HBB基因表达量的变化倍数最大,下调约98倍。差异表达基因的富集分析结果表明,哈萨克羊尾脂中上调表达的基因主要参与脂肪酸代谢过程。另外,在2个品种尾脂中鉴定出11 870个可变剪接事件,其中383个为差异剪接事件。对相关差异表达基因和可变剪接的进一步研究将有助于揭示绵羊肥脂尾形成的分子机制。
[Abstract]:To identify differentially expressed genes and variable splicing in tail fat tissue of Kazak sheep and Tibetan sheep, Two high-throughput sequencing libraries were constructed by extracting 6 unrelated total RNAs from tail adipose tissue of 2 year old adult Kazak sheep and Tibetan sheep. The short sequences were mapped to the sheep reference genome by TopHat2 software. Cuffdiff software was used to analyze gene expression abundance and differentially expressed genes. The abundance of 19 genes in tail fat of two breeds was higher than that of 1 000 FPKM, including ADIPOQN Sparc vim and so on. Among them, the transcripts with the highest expression in tail adipose tissue of 2 breeds were located in mitochondrial genome 5331n14154bp. To include such genes as COX1, COX2, COX2, ATP 8, The expression abundance of FABP4 in Kazak sheep and Tibetan sheep was as high as 29 724.20 and 28 818.80 FPKM, respectively, while FABP4 was the most abundant nuclear gene. The expression levels in Kazak sheep and Tibetan sheep were 3 194.03 and 2 667.09 respectively. A total of 627 differentially expressed genes were identified by differential expression gene analysis, which were compared with those in fat tissue of Tibetan sheep tail. There were 221 up-regulated genes and 406 down-regulated genes in tail adipose tissue of Kazak sheep. The up-regulated genes in Kazak tail fat were mainly involved in fatty acid metabolism. In addition, 11,870 variable splicing events were identified in two varieties of tail fat. 383 of them were differential splicing events. Further study of differentially expressed genes and variable splicing will help to reveal the molecular mechanism of fat tail formation in sheep.
【作者单位】：四川农业大学动物科技学院;
【基金】：四川省教育厅项目(15ZB0005)~~
【分类号】：S826

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