miR-338基因簇与食管癌发病风险的病例对照研究

发布时间：2018-02-27 19:42

本文关键词： mi R- 基因簇食管癌生物标志物　出处：《癌变·畸变·突变》2016年02期 　论文类型：期刊论文

【摘要】：目的:探讨mi R-338基因簇在食管癌组织中的表达模式及其与食管癌发病风险的关系。方法:选取86例经病理确诊的食管癌患者,分别提取食管癌和癌旁组织总RNA,采用实时荧光定量PCR法检测hsa-mi R-338-3p、hsa-mi R-338-5p与hsa-mi R-657的表达,应用配对样本t检验分析癌和癌旁组织中mi RNA表达差异,Pearson相关分析检验基因簇各mi RNA表达的相关性,logistic回归分析mi RNA异常表达对食管癌发病风险的影响。结果:hsa-mi R-338-3p与hsa-mi R-338-5p在食管癌组织中的表达均较癌旁组织降低(P均0.05),hsa-mi R-657在食管癌和癌旁组织中表达的差异无统计学意义(P0.05),hsa-mi R-338-3p与hsa-mi R-338-5p表达显著相关(r=0.754,P0.05),hsa-mi R-338-5p的低表达增加了食管癌的发病风险(OR=1.264,P0.05),可能是食管癌的危险因素。结论:mi R-338基因簇可能抑制了食管癌的发生,hsa-mi R-338-5p可能成为食管癌诊断的潜在标志物。
[Abstract]:Objective: to investigate the expression pattern of miR-338 gene cluster in esophageal carcinoma and its relationship with the risk of esophageal cancer. Methods: 86 patients with esophageal cancer were selected. The total RNAs of esophageal carcinoma and adjacent tissues were extracted, and the expression of hsa-mi R-338-3psa-mi R-338-5p and hsa-mi R-657 were detected by real-time fluorescence quantitative PCR. The difference of mi RNA expression in cancer and paracancerous tissues was analyzed by paired t-test. Pearson correlation analysis was used to test the correlation between the expression of mi RNA in gene clusters and the correlation between the expression of mi RNA. The risk of esophageal cancer was analyzed by using the abnormal expression of mi RNA. Results: hsa-mi R-338-3p. The expression of hsa-mi R-338-5p in esophageal carcinoma was significantly lower than that in paracancerous tissues. There was no significant difference in the expression of hsa-mi R-657 between esophageal carcinoma and adjacent tissues. There was no significant correlation between the expression of hsa-mi R-338-3p and the expression of hsa-mi R-338-5p. The low expression of R0.754P0.05HSA-mi R-338-5p increased the expression of HSA-mi R-338-5p in esophageal carcinoma. It may be a risk factor for esophageal cancer. Conclusion the gene cluster of 1: mi R-338 may inhibit the occurrence of esophageal carcinoma and may be a potential marker for the diagnosis of esophageal carcinoma.
【作者单位】：东南大学公共卫生学院环境医学工程教育部重点实验室;
【基金】：国家自然科学基金(81172747,81573108,81573191)
【分类号】：R735.1

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