小立碗藓PpAGO7基因的克隆及表达分析

发布时间：2018-02-27 22:42

  本文关键词： 小立碗藓 PpAGO 基因克隆 表达分析　出处：《西北植物学报》2017年08期 　论文类型：期刊论文

【摘要】：该研究采用同源克隆的方法获得了小立碗藓AGO7蛋白编码基因PpAGO7,对PpAGO7基因序列特征进行生物信息学分析,并利用Real-time PCR方法分析PpAGO7基因的时空表达模式,为揭示小立碗藓中PpAGO7基因的生物学功能提供依据。结果表明:PpAGO7基因的全长cNDA为3 583bp,其中开放阅读框3 363bp,编码1 120个氨基酸,分子量123.38kD,等电点9.26,含有AGO蛋白典型的PAZ和PIWI结构域;PpAGO7基因在小立碗藓整个生活周期都有表达,且在茎叶体生长时期表达水平较高,但在原丝体时期表达水平较低。研究结果推测PpAGO7基因可能在茎叶体拟叶的生长发育过程中起作用。
[Abstract]:In this study, AGO7 protein encoding gene PpAGO7 was obtained by homologous cloning. The sequence of PpAGO7 gene was analyzed by bioinformatics, and the temporal and spatial expression pattern of PpAGO7 gene was analyzed by Real-time PCR method. The results showed that the full length cNDA of the PpAGO7 gene was 3 583bp, and the open reading frame was 3 363bp, encoding 1 120 amino acids. Molecular weight 123.38kD, isoelectric point 9.26. the PAZ and PIWI domain PpAGO7, which contains AGO protein, was expressed in the whole life cycle of Moss, and the expression level of PpAGO7 was higher during the growth of stem and leaf. The results suggested that PpAGO7 gene might play an important role in the growth and development of stem and leaf mimicry.
【作者单位】： 江苏师范大学生命科学学院;苏州大学电子信息学院;
【基金】：基金项目:2015年江苏省研究生创新工程(KYZZ15-0388) 江苏省高校优势学科建设工程
【分类号】：Q943.2
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本文编号：1544629