红掌2个SOC1基因的克

发布时间：2018-02-27 23:40

本文关键词： 红掌 SOC基因生物信息学表达分析　出处：《浙江大学学报(农业与生命科学版)》2017年03期 　论文类型：期刊论文

【摘要】：为了明确SOC1转录因子在红掌中的成员及作用机制,采用5'/3'末端快速扩增技术获得这2个SOC1基因的完整编码区,通过生物信息学软件分析这2个基因的相关信息,并结合荧光定量反转录-聚合酶链式反应验证这2个基因的表达模式。结果显示:获得的2个基因核苷酸长度分别为651 bp和642 bp,命名为Aa SOC1-1和Aa SOC1-2;2个转录因子在二级结构上均有螺旋、折叠和转角,未发现其他结构。系统进化分析显示,二者与单子叶植物同类蛋白距离较近,与红掌的植物学分类地位一致。表达分析结果显示,2个基因均在营养器官和生殖器官中表达,但表达水平略有差异。研究认为,从红掌中克隆的2个SOC1转录因子可能定位于线粒体而非细胞核中,二者在序列组成和表达模式上既有保守性也存在一定的差异性,预示它们在功能上可能也存在一定的区别。
[Abstract]:In order to clarify the membership and mechanism of SOC1 transcription factors in the palm of red palm, the complete coding regions of the two SOC1 genes were obtained by rapid amplification of 5 / 3 'terminal, and the related information of the two genes was analyzed by bioinformatics software. The expression patterns of these two genes were confirmed by fluorescence quantitative reverse transcription-polymerase chain reaction. The results showed that the nucleotide lengths of the two genes were 651bp and 642bprespectively, named AA SOC1-1 and AaSOC1-2. There are helix in the secondary structure, No other structures were found at folding and turning angles. Phylogenetic analysis showed that they were closer to monocotyledonous proteins. The results of expression analysis showed that the two genes were expressed in vegetative and reproductive organs, but the level of expression was slightly different. The two SOC1 transcription factors cloned from the red palm may be located in the mitochondria but not in the nucleus. There are both conserved and different sequence composition and expression patterns between them, indicating that there may be some differences in their functions.
【作者单位】：浙江省农业科学院花卉研究开发中心;浙江省杭州萧山技师学院;
【基金】：国家自然科学基金(31200527) 浙江省农业新品种选育重大专项(2016C02056-13)
【分类号】：Q943.2;S682.14

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