中风病痰湿体质患者载脂蛋白E基因多态性分布特点研究

发布时间：2018-02-28 06:41

本文关键词： 中风病痰湿体质载脂蛋白E 基因多态性血脂　出处：《中医杂志》2017年15期 　论文类型：期刊论文

【摘要】：目的探讨中风病痰湿体质与载脂蛋白E(ApoE)基因多态性之间的相关性。方法纳入中风病痰湿体质患者77例(观察组),中风病非痰湿体质患者80例(对照组)。收集患者的一般资料,检测血脂及血清载脂蛋白水平,包括甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、载脂蛋白A1(ApoA1)、载脂蛋白B(ApoB)、脂蛋白(a)[Lp(a)]、ApoE水平,并检测ApoE基因多态性。结果与对照组比较,观察组血清中TC、TG、LDL-C、ApoE、ApoB水平均升高,HDL-C水平及ApoA1/ApoB降低(P0.05或P0.01),而Lp(a)、ApoA1水平比较差异均无统计学意义(P0.05)。共检出3种基因型( ε3/ ε3、ε2/ ε3、ε3/ ε4)和3种等位基因( ε2、ε3、ε4)。两组基因型及等位基因分布经哈迪-温伯格平衡检验比较示差异无统计学意义(P0.05),符合哈迪-温伯格遗传平衡。两组ApoE基因型及其等位基因整体分布情况差异均有统计学意义(P0.05)。与对照组比较,观察组携带ApoE ε4等位基因及ApoE ε3/ ε4基因型患者较多(P0.05),其余基因型及等位基因在两组间的分布差异均无统计学意义(P0.05)。结论中风病痰湿体质易患中风可能与携带ApoE ε4等位基因、ApoE ε3/ ε4基因型有关,痰湿体质者血脂浓度较高可能受ApoE基因多态性调控。
[Abstract]:Objective to investigate the relationship between phlegm dampness constitution and apolipoprotein ApoE gene polymorphism in apoplexy. Methods 77 patients with phlegm dampness constitution of apoplexy (observation group) and 80 patients with apoplectic non-phlegm wet constitution (control group) were included in this study. Blood lipid and serum apolipoprotein levels, including triglyceride TGN, total cholesterol TCU, high density lipoprotein cholesterol (HDL-CU), low density lipoprotein cholesterol (LDL-CU), apolipoprotein A1A1 (ApoA1), apolipoprotein B (ApoBN), lipoprotein (ApoA) [Lpa-] ApoE, were measured. The polymorphism of ApoE gene was detected. In the observation group, the levels of HDL-C and ApoA1/ApoB decreased P05 or P0.01, but there was no significant difference in the levels of ApoA1 between the two groups. Three genotypes (蔚 3 / 蔚 3, 蔚 2 / 蔚 3, 蔚 3 / 蔚 4) and three alleles (蔚 2, 蔚 3, 蔚 4) were detected. The distribution of ApoE genotypes and alleles in the two groups were significantly different from those in the control group. There was no significant difference in the distribution of ApoE genotypes and alleles between the two groups by the Hardy Weinberg equilibrium test (P 0.05), which was consistent with the Hardy Weinberg genetic balance, and there were significant differences between the two groups in the distribution of ApoE genotypes and alleles. The patients with ApoE 蔚 4 allele and ApoE 蔚 3 / 蔚 4 genotype were more likely to carry ApoE 蔚 4 allele than those with ApoE 蔚 3 / 蔚 4 genotype. There was no significant difference in the distribution of other genotypes and alleles between the two groups. Conclusion the phlegm dampness constitution of apoplexy is likely to suffer from stroke and carry ApoE 蔚 4. The allele ApoE 蔚 3 / 蔚 4 was related to the genotype of ApoE 蔚 3 / 蔚 4. The higher blood lipid concentration in phlegm and dampness constitution may be regulated by ApoE gene polymorphism.
【作者单位】：福建中医药大学护理学院;福建中医药大学附属康复医院;
【基金】：福建省自然科学基金(2014J01350)
【分类号】：R255.2

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