当归挥发油对自发性高血压大鼠miR-155及其靶基因的影响

发布时间：2018-02-28 11:19

  本文关键词： 高血压 当归挥发油 miR- 血管紧张素Ⅱ型受体拮抗剂　出处：《中国全科医学》2017年09期 　论文类型：期刊论文

【摘要】：目的探究当归挥发油对自发性高血压大鼠(SHR)miR-155及其靶基因的影响,并从肾素-血管紧张素-醛固酮系统(RAAS)探讨当归挥发油对SHR血压的改善作用及其机制。方法 2015年10月—2016年4月,将8周龄48只SPF级雄性SHR随机分为模型组、当归挥发油低剂量组、当归挥发油中剂量组、当归挥发油高剂量组、藁本内酯组、缬沙坦组,各8只;另选取8只SPF级正常血压雄性Wistar大鼠作为正常组。正常组、模型组不给予任何药物,当归挥发油低剂量组、当归挥发油中剂量组、当归挥发油高剂量组、藁本内酯组、缬沙坦组大鼠分别灌胃给予当归挥发油乳剂12.6 mg·kg~(-1)·d~(-1)、37.0 mg·kg~(-1)·d~(-1)、100.0 mg·kg~(-1)·d~(-1),藁本内酯溶液0.8 mg·kg~(-1)·d~(-1),缬沙坦溶液51.4 mg·kg~(-1)·d~(-1),连续给药4周,给药期间各组大鼠标准饮食、自由饮水。检测给药前及给药第1、2、3、4周各组大鼠收缩压变化。灌胃4周后处死大鼠取心脏,HE染色观察心肌组织病理学的变化,采用Western blotting法检测血管紧张素Ⅱ1型受体(AT1R)、细胞外调节蛋白激酶1/2(ERK1/2)、磷酸化ERK1/2(p-ERK1/2)表达水平。采用RT-qPCR法检测miR-155表达水平。结果不同处理情况、时间在大鼠收缩压中存在交互作用(P0.05);不同处理情况、时间在大鼠收缩压中主效应显著(P0.05)。给药前及给药第1、2、3周,模型组、当归挥发油低剂量组、当归挥发油中剂量组、当归挥发油高剂量组、藁本内酯组、缬沙坦组大鼠收缩压均高于正常组(P0.05);给药第4周模型组大鼠收缩压高于正常组(P0.05);给药前当归挥发油低剂量组、当归挥发油中剂量组、当归挥发油高剂量组、藁本内酯组、缬沙坦组大鼠收缩压均高于模型组,给药第1、2、3、4周当归挥发油低剂量组、当归挥发油中剂量组、当归挥发油高剂量组、藁本内酯组、缬沙坦组大鼠收缩压均低于模型组(P0.05)。HE染色显示,模型组大鼠心肌组织红细胞多而挤,心肌横纹不清,提示心肌充血,右心功能受损;当归挥发油低、中、高剂量组大鼠心肌组织出现横纹且清晰。当归挥发油低剂量组、当归挥发油中剂量组、当归挥发油高剂量组、藁本内酯组、缬沙坦组大鼠AT1R、ERK1/2、p-ERK1/2表达水平均低于模型组(P0.05)。7组大鼠miR-155表达水平比较,差异无统计学意义(P0.05)。结论当归挥发油能够明显抑制AT1R基因的表达,通过调节ERK1/2信号通路从而达到降压的功能;当归挥发油不通过miR-155在心肌组织发挥作用。
[Abstract]:Objective to investigate the effect of volatile oil of Angelica sinensis on SHR-miR-155 and its target gene in spontaneously hypertensive rats. The effects of essential oil of Angelica sinensis on SHR blood pressure and its mechanism were studied from the renin angiotensin-aldosterone system. Methods from October 2015 to April 2016, 48 SPF grade male SHR aged 8 weeks were randomly divided into model group. Low dose group, middle dose group, high dose group, ligustilide group, valsartan group, and 8 male Wistar rats with normal blood pressure of SPF grade were selected as normal group. The model group was not given any drugs, the low dose group, the middle dose group, the high dose group, the ligustilide group, the middle dose group, the ligustilide group, the middle dose group, the high dose group, and the ligustilide group, respectively. The rats in valsartan group were given Angelica volatile oil emulsion 12.6 mg 路kg ~ (-1) 路d ~ (-1) ~ (-1) ~ (37.0 mg 路kg ~ (-1)) ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1)) by gavage, ligustilide solution (0.8 mg 路kg ~ (-1) ~ (-1)) 路d ~ (-1) ~ (-1), valsartan solution (51.4 mg 路kg ~ (-1)) 路d ~ (-1) 路L ~ (-1) for 4 weeks, and during the period of drug administration, rats in each group were fed with a standard diet of 0.80 mg 路kg ~ (-1) 路kg ~ (-1) 路L ~ (-1). Free drinking water. The changes of systolic blood pressure (SBP) were detected before administration and 4 weeks after administration. After 4 weeks of gastric perfusion, the rats were sacrificed to observe the pathological changes of myocardium by HE staining. The expression levels of angiotensin 鈪，

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