西藏牦牛源大肠杆菌主要耐药表型及耐药基因检测

发布时间：2018-02-28 13:10

本文关键词： 西藏牦牛大肠杆菌 rmtB flor sul　出处：《中国兽医学报》2017年08期 　论文类型：期刊论文

【摘要】：对西藏6个不同地、市200株牦牛源大肠杆菌进行了氨基糖苷类、氟苯尼考和磺胺类药物耐药表型和耐药基因的PCR检测。结果显示:牦牛源大肠杆菌对氨基糖苷类药物耐药率分别为阿米卡星96%、链霉素94.59%、新霉素19%、庆大霉素23%、卡那霉素19%,耐药基因只检出rmtB基因,检出率为100%;氟苯尼考类药物耐药率为25%,耐药基因flor基因检出率25%;磺胺类药物耐药率为32%,耐药基因sul1基因检出率7%、sul2基因检出率7%、sul3基因检出率17%;且耐药表型与耐药基因检测结果基本一致,同时显示,西藏不同地区间牦牛大肠杆菌的耐药性存在差异,人口密集和流动较大的拉萨、林芝、日喀则市耐药现象较为严重,而阿里、山南和那曲地区相对较轻。以上耐药表型和耐药基因在西藏不同市、地区均有检出,同时存在多重耐药现象。结果表明,西藏牦牛源大肠杆菌存在耐药性,应引起重视,提示西藏地区要合理用药,减轻耐药性的产生。
[Abstract]:Aminoglycosides of 200 yak Escherichia coli strains from 6 different cities in Tibet were studied. PCR detection of florfenicol and sulfanilamide resistance phenotypes and resistance genes. The results showed that the resistance rates of Escherichia coli to aminoglycosides from yak were amicacin 96, streptomycin 94.59, neomycin 19, gentamicin 23 and kanax, respectively. The drug resistance gene only detects the rmtB gene. The detectable rate of florfenicol was 25 and the detection rate of resistant gene flor was 25. The rate of sulfadiazine resistance was 32. The detection rate of sul1 gene was 7. The detection rate of sul2 gene was 7. The detection rate of sul3 gene was 17. The phenotypic and resistance groups of florfenicol were 17. Because the results of the tests are basically the same, At the same time, there are differences in drug resistance among yak Escherichia coli in different regions of Tibet. Lhasa, Lhasa, Linzhi, and Xigaze City, where the population is dense and mobile, are more serious, but Ali, In Shannan and Naqu areas, the resistance phenotypes and resistance genes were detected in different cities and regions of Tibet. The results showed that Escherichia coli from Tibetan yaks had drug resistance, which should be paid more attention to. It is suggested that rational drug use should be used in Tibet to reduce the occurrence of drug resistance.
【作者单位】：西藏大学农牧学院动物科学学院;
【基金】：国家肉牛牦牛产业体系资助项目(CARS-38) 国家自然科学基金资助项目(31160514) 西藏自治区科技厅重点资助项目
【分类号】：S852.612

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