兔眼蓝莓组培红色突变株CHS基因的克隆与分析

发布时间：2018-02-28 14:21

本文关键词： 生物信息学兔眼蓝莓组织培养 CHS 花青素　出处：《浙江农林大学学报》2017年05期 　论文类型：期刊论文

【摘要】：蓝莓Vaccinium spp.果实含有丰富的花青素,但目前对蓝莓花青素合成相关基因的研究还较少。为分析蓝莓花青素合成相关基因,也为今后高花青素含量品种的育种工作提供一定的生物信息学基础,以红色突变兔眼蓝莓Vaccinium ashei为试验材料,采用同源克隆方法 ,克隆到查尔酮合成酶基因(CHS)的cDNA全长。生物信息学分析表明:该基因cDNA全长1 398 bp,有1个1 170 bp的完整开放阅读框(ORF),编码389个氨基酸。与杜鹃Rhododendron simsii,山茶Camellia japonica,红花油茶Camellia chekiangoleosa,中华猕猴桃Actinidia chinensis,高丛蓝莓Vaccinium corymbosum,苹果Malus domestica的CHS基因同源性分别为90%,83%,83%,83%,83%与82%。兔眼蓝莓CHS基因编码的蛋白不存在信号肽,无跨膜结构域,定位于细胞质中,肽链表现为疏水性,α-螺旋和随机卷曲是其蛋白质二级结构中量最大的结构元件,β-转角和β-折叠散布于整个蛋白质中,对编码的蛋白的结构域预测显示该蛋白含有类似查尔酮合成酶超家族(CHS-like超家族)结构域。由此推测:兔眼蓝莓CHS基因是高度保守的,表达的蛋白为疏水性蛋白,是一种结构和性质都相对稳定的蛋白。
[Abstract]:The fruits of blueberry Vaccinium spp. are rich in anthocyanins, but there are few studies on the genes related to blueberry anthocyanin biosynthesis in order to analyze the genes related to blueberry anthocyanin synthesis. It also provides a certain basis of bioinformatics for breeding varieties with high anthocyanin content. Using Vaccinium ashei of red mutant rabbit eye as experimental material, homologous cloning method was used. CDNA was cloned into Chalcone synthase gene. Bioinformatics analysis showed that the full length of cDNA was 1 398 BP, with a complete open reading frame of 1 170 BP, encoding 389 amino acids. The sequence was compared with Rhododendron simsii, Camellia japonica and Carthamus japonica. The homology of CHS gene of Camellia chekiangoleosa, Actinidia chinensis, Vaccinium corymbosum and Malus domestica were 90.83% and 833% respectively. The protein encoded by CHS gene of blueberry did not contain signal peptide. The peptide chain is hydrophobic. 伪 -helix and random crimp are the largest structural elements in the secondary structure of the protein. The 尾 -rotation angle and 尾 -fold are scattered in the whole protein. The prediction of the domain of the encoded protein indicates that the protein contains a domain similar to the Chalcone synthase superfamily (CHS-like superfamily). It is inferred that the CHS gene of blueberry is highly conserved and the expressed protein is hydrophobic. It is a protein with relatively stable structure and properties.
【作者单位】：西南林业大学云南省高校林木遗传改良与繁育重点实验室;
【基金】：云南省林学一流学科建设资助项目(51600625) 云南省高校林木遗传改良与繁育重点实验室开放基金资助项目(31360404)
【分类号】：S663.9

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