水稻矮秆突变体htr的表型分析及基因克隆

发布时间：2018-03-01 17:00

本文关键词： 水稻矮秆基因克隆转录因子 AP　出处：《南京农业大学学报》2017年04期 　论文类型：期刊论文

【摘要】：[目的]对水稻矮秆突变体htr进行表型分析与基因克隆,为水稻育种提供新的矮秆基因资源。[方法]对矮秆突变体htr茎秆进行细胞学观察,并将htr与‘N22’和‘9311’杂交构建F2群体,提取隐性极端个体定位基因HTR。[结果]htr是一个稳定遗传的矮秆突变体,其株高、穗长、千粒质量、分蘖数等性状均降低,但其叶片变宽,叶色深绿,茎秆增粗。成熟期对htr倒二节间进行切片观察发现:相比‘9311’,htr在茎秆的纵轴方向细胞未能正常伸长,横向细胞数目增多,细胞变小。遗传分析表明突变体htr的矮秆性状受1对隐性单基因控制。利用F2群体中隐性极端个体将HTR定位在第5染色体长臂ttr-1和ttr-2两个分子标记之间,物理距离大约在144 kb范围内。测序结果发现htr中编码AP2转录因子(AP2-like ethylene-responsive transcription factor)基因发生点突变,导致保守氨基酸发生替换,亚细胞定位显示HTR定位于细胞核中。HTR呈现组成型表达,但以在茎秆中表达量最高。[结论]矮秆突变体htr突变性状受一对编码AP2转录因子的隐性单基因控制。HTR基因的克隆对培育厚壁粗秆抗倒伏水稻新品种提供了一定的理论参考。
[Abstract]:[objective] to study the phenotypic analysis and gene cloning of rice dwarf mutant htr, and to provide a new dwarf gene resource for rice breeding. [methods] the stem of dwarf mutant htr was observed by cytological observation, and the F 2 population was constructed by crossing htr with N22 'and Y9311'. [results] htr was a stably inherited dwarf mutant. Its plant height, ear length, thousand grain mass, tiller number and other traits were all decreased, but the leaves became wider and the leaves were dark green. The results showed that the cells in the longitudinal axis of the stem were not normally elongated, and the number of the transverse cells was increased, compared with 9311htr in the vertical axis of the stem, and the number of the cells in the lateral direction was increased, compared with that in the vertical axis of the stem. Genetic analysis showed that the dwarf traits of the mutant htr were controlled by a pair of recessive single genes. HTR was located between the long arm ttr-1 and ttr-2 markers of chromosome 5 by using recessive extreme individuals in F2 population. The physical distance was about 144kb. The results of sequencing showed that the gene encoding AP2 transcription factor AP2-like ethylene-responsive transcription factor occurred point mutation in htr, resulting in the substitution of conserved amino acids, and subcellular localization showed that HTR was localized in the nucleus. [conclusion] the cloning of htr gene controlled by a pair of recessive single genes encoding AP2 transcription factors may provide a theoretical reference for the cultivation of new rice varieties with thick stem resistance to lodging.
【作者单位】：南京农业大学作物遗传与种质创新国家重点实验室;
【基金】：国家重点研发项目七大农作物育种专项(2016YFD0100101-08) 国家863计划项目(2014AA10A603-15) 江苏省科技支撑计划项目(BE2014394,BE2015363) 江苏省农业科技自主创新资金课题(CX(16)1029)
【分类号】：S511

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