辽宁省朝阳市细粒棘球病的基因型分析

发布时间：2018-03-01 20:02

本文关键词： 细粒棘球蚴 cox基因基因型分子流行病学　出处：《中国人兽共患病学报》2017年02期 　论文类型：期刊论文

【摘要】：目的分析本院分离到的人源性棘球蚴的基因型,为辽宁省棘球蚴病的分子流行病学提供资料。方法经外科手术方法从患者肝内获取棘球蚴囊,将囊内容物离心沉淀提取DNA,以细粒棘球绦虫线粒体细胞色素氧化酶(cox1)基因为靶基因设计引物进行PCR扩增,对PCR产物进行测序,测序结果应用Blast进行分析,并与GenBank数据库中序列进行比对。结果 cox1扩增产物及测序的结果提示,本例样本与细粒棘球幼的cox1基因型(AF297617)高度相似,差异性为0.1%,基因型属于G1亚型。并与其他国家的25个分离株序列一致。结论线粒体基因组的cox1基因分析是棘球蚴病诊断和分型的有力工具。辽宁地区首次报道了细粒棘球绦虫的基因型为G1亚型,其序列与国内外的多个分离株保持一致,为棘球蚴病的分子流行病学补充了资料。
[Abstract]:Objective to analyze the genotypes of human echinococci isolated from our hospital and to provide data for molecular epidemiology of echinococcosis in Liaoning Province. The DNA was extracted by centrifugation precipitation from the capsule contents. The PCR products were amplified by PCR using the mitochondrial cytochrome oxidase cox1) as the target gene of Echinococcus granulosus. The PCR products were sequenced. The results were analyzed by Blast. Results the results of cox1 amplification and sequencing showed that the sample was highly similar to the cox1 genotype AF297617 of young spinous granulosus. The difference is 0.1 and the genotype belongs to G1 subtype. It is consistent with 25 isolates from other countries. Conclusion cox1 gene analysis of mitochondrial genome is a powerful tool for diagnosis and typing of echinococcosis. The genotype of Echinococcus granulosus was G1 subtype. The sequence is consistent with many isolates at home and abroad, which provides additional data for molecular epidemiology of echinococcosis.
【作者单位】：中国医科大学附属第一医院内分泌科内分泌研究所辽宁省内分泌疾病重点实验室;中国医科大学塔城医院消化内分泌科;中国医科大学基础医学院病原生物学教研室;
【基金】：国家自然科学基金(No.81370189);国家自然科学基金青年基金(No.81302271)联合资助~~
【分类号】：R532.32

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