两近缘种茶尺蠖的抗菌肽基因在抵抗EoNPV感染过程中的表达差异

发布时间：2018-03-02 19:07

本文选题：灰茶尺蛾　切入点：小茶尺蠖　出处：《中国生物防治学报》2017年04期 　论文类型：期刊论文

【摘要】：小茶尺蠖Ectropis obliqua Prout和灰茶尺蛾Ectropis grisescens Warren是危害茶园的两近缘种害虫。用小茶尺蠖核型多角体病毒E.obliqua nucleopolyhedrovirus(Eo NPV)喂饲两近缘种茶尺蠖,10 d后小茶尺蠖的死亡率为100%,而灰茶尺蛾的死亡率仅为35.5%。以感染病毒的两近缘种3龄幼虫的cDNA为模板,采用RT-PCR技术克隆获得了gloverin、attacin和moricin 3个抗菌肽基因,通过邻位连接法构建系统发育树聚类分析显示小茶尺蠖和灰茶尺蛾的Gloverin、Attacin和Moricin蛋白均分别归为一个分支;进一步采用qPCR检测发现,病毒处理后gloverin、attacin和moricin 3个基因在两近缘种茶尺蠖体内均表达上调,gloverin基因在12 h达到最大值,moricin基因在48 h达到最大值,且gloverin和moricin基因在灰茶尺蛾中表达量显著高于小茶尺蠖,而attacin基因在病毒侵入后的不同时期在两近缘种茶尺蠖体内表达量是变化的,推测gloverin、moricin和attacin基因可能与病毒的侵入有关。这一研究结果为进一步揭示抗菌肽基因在昆虫与病毒互作中的作用打下了基础。
[Abstract]:Ectropis obliqua Prout and Ectropis grisescens Warren are two related species pests that harm tea plantations. The death rate of two closely related species, E. obliqua nucleopolyhedrovirus(Eo NPV, was 100 and 100 after 10 days feeding with E. obliqua nucleopolyhedrovirus(Eo NPV. The mortality rate was only 35.5.The cDNA of the third instar larvae of two related species infected with the virus was used as a template. Three antimicrobial peptide genes, gloverin attacin and moricin, were cloned by RT-PCR technique. The phylogenetic tree cluster analysis showed that Gloverin attacin and Moricin proteins were classified into one branch, respectively, and were further detected by qPCR. After virus treatment, the expression of gloverin and moricin genes reached the maximum at 12 h, and the expression of gloverin and moricin genes was significantly higher than that of small tea geometrid in two related species of tea geometrid, the expression of gloverin gene reached the maximum at 12 h and the expression of moricin gene reached the maximum at 48 h. However, the expression of attacin gene changed in two relative species of tea geometrid at different time after virus invasion. It is suggested that gloverinmoricin and attacin genes may be related to the invasion of the virus. This study provides a basis for further understanding the role of antimicrobial peptide gene in the interaction between insect and virus.
【作者单位】：中国农业科学院茶叶研究所;
【基金】：国家科技支撑计划(2011BAD01B02) 中国农业科学院科技创新工程(CAAS-ASTIP-2016-TRICAAS)
【分类号】：S435.711

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