藏鸡GHRHR-v1基因克隆及组织表达谱研究

发布时间：2018-03-02 18:26

本文选题：藏鸡　切入点：GHRHR-v　出处：《中国家禽》2017年10期 　论文类型：期刊论文

【摘要】：为分析生长激素释放激素受体剪接突变体(GHRHR-v1)基因结构及在各个组织中表达水平在藏鸡生长发育中的特性,以150日龄藏鸡为研究对象,利用RT-PCR技术克隆藏鸡GHRHR-v1基因并进行序列测定,采用q PCR技术检测GHRHR-v1基因在藏鸡不同组织中的表达水平,从而构建该基因的组织表达谱。结果表明:GHRHR-v1基因序列长度为1 279 bp,其中开放阅读框长度为1 260 bp,编码419个氨基酸,藏鸡与鸡GHRHR-v1基因(登录号为DQ230840)核苷酸的同源性为99.76%,预测的氨基酸序列同源性为100%,两者亲缘关系最近。组织表达谱结果表明,GHRHR-v1基因在藏鸡心脏、肝脏、脾脏、肺脏、肾脏、胸肌、腿肌、下丘脑和垂体中均有表达,在垂体中表达水平最高,高表达组织为心脏、肝脏、脾脏和肺脏,低表达组织为肾脏、胸肌、腿肌和下丘脑,说明GHRHR-v1基因的表达主要集中于垂体。该结果为进一步研究藏鸡下丘脑-垂体生长轴上相关候选基因对生长发育的调控及藏鸡的选育提供一定的理论依据。
[Abstract]:In order to analyze the gene structure of growth hormone releasing hormone receptor splicing mutant (GHRHR-v1) and its expression level in various tissues, 150 days old Tibetan chickens were studied. The GHRHR-v1 gene of Tibetan chicken was cloned by RT-PCR and sequenced. The expression level of GHRHR-v1 gene in different tissues of Tibetan chicken was detected by Q PCR technique. The results showed that the length of the gene was 1 279 BP, and the open reading frame length was 1 260 BP, encoding 419 amino acids. The homology of nucleotide sequence of GHRHR-v1 gene (accession number DQ230840) was 99.76 and predicted amino acid sequence was 100. The result of tissue expression profile showed that GHR-v1 gene was present in heart, liver, spleen, lung, kidney, chest muscle of Tibetan chicken. The expression level of leg muscle, hypothalamus and pituitary gland was the highest, the high expression tissues were heart, liver, spleen and lung, and the low expression tissues were kidney, chest muscle, leg muscle and hypothalamus. The results provided a theoretical basis for further study on the regulation of growth and development by candidate genes on the hypothalamus-pituitary axis of Tibetan chickens and the breeding of Tibetan chickens.
【作者单位】：西南民族大学青藏高原研究院;西南民族大学生命科学与技术学院;
【基金】：四川省科技支撑计划项目(2016NYZ0043) 西南民族大学研究生学位点建设项目(CX2017SZ031)
【分类号】：S831.2

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