太子参玉米黄质环氧化酶基因的克隆与表达分析

发布时间：2018-03-03 19:11

本文选题：太子参　切入点：玉米黄质环氧化酶　出处：《中国中药杂志》2017年04期 　论文类型：期刊论文

【摘要】：玉米黄质环氧化酶(zeaxanthin epoxidase,ZEP)在植物脱落酸(abscisic acid,ABA)生物合成间接途径中发挥着重要调节作用。该研究根据太子参转录组数据,克隆获得太子参的Pseudostellaria heterophylla玉米黄质环氧化酶基因,命名Ph ZEP。对其序列进行生物信息学分析,结果表明Ph ZEP的开放阅读框(open reading frame,ORF)为1 263 bp,编码420个氨基酸,预测其蛋白的相对分子质量为47.34 k Da,等电点(theoretical p I)为6.64;具有脂质蛋白附着点和黄素蛋白单加氧酶的特征性结构域,且在N端含有分泌信号肽。实时荧光PCR分析发现,Ph ZEP在叶片中表达量最高,其次是茎和须根;Ph ZEP在盛花期后10,40 d块根中的表达量较高;ABA处理后Ph ZEP表达量与对照组相当,经氟啶酮处理后Ph ZEP的表达量显著高于对照组。该研究从太子参中分离鉴定了ZEP基因,对后续研究其基因的功能特点和解析ABA在太子参生长发育过程的遗传调控提供研究基础。
[Abstract]:Zeaxanthin epoxidase (ZEP) plays an important role in the indirect biosynthesis pathway of plant abscisic acididase (Aba). Based on the transcriptional data of Pseudostellaria heterophylla, the Pseudostellaria heterophylla zeaxanthin xidase gene was cloned. The sequence of Ph ZEP. was analyzed by bioinformatics. The results showed that the open reading frame of Ph ZEP was 1 263 BP, encoding 420 amino acids. The predicted molecular weight of the protein is 47.34 kDa and the isoelectric point is 6.64, which has the characteristic domain of lipid protein attachment point and Flavin monooxygenase. The expression of Ph ZEP was the highest in leaves, followed by the expression of Ph ZEP in stem and fibrous root at 10 ~ 40 days after flowering. The expression of Ph ZEP in stem and fibrous root was similar to that in control group. The expression of Ph ZEP was significantly higher than that of the control group. The ZEP gene was isolated and identified from Pseudostellaria heterophylla in this study, which provided a basis for further study on the functional characteristics of the gene and the genetic regulation of ABA in the growth and development of Pseudostellaria heterophylla.
【作者单位】：贵阳中医学院;
【基金】：国家自然科学基金项目(81460579,81160501) 贵州省研究生工作站建设项目(黔教研合JYSZ字[2014]016) 施秉中药材产业科技合作专项计划项目[施中药科合专项(2014)第6号]
【分类号】：S567.53

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