大叶落地生根类糖原合成激酶KdSK基因的克隆与表达分析

发布时间：2018-03-04 22:12

本文选题：大叶落地生根　切入点：类糖原合成酶激酶　出处：《广西植物》2017年08期 　论文类型：期刊论文

【摘要】：类糖原合成酶激酶(SKs)属于丝氨酸/苏氨酸类蛋白激酶,在植物器官发育、激素信号传导过程中十分重要,并参与生物胁迫、非生物胁迫的应答过程。大叶落地生根中的胎生苗发育过程,同时具备胚胎发生和器官发生的特征,是研究无性生殖的理想模型。为了更好地理解大叶落地生根中胎生苗发育的分子机制,该研究利用RACE-PCR技术,从大叶落地生根中克隆了1个新的基因KdSK。该基因具有423个氨基酸残基,分子量为47.79 kD,等电点为8.37,其开放阅读框长为1 272 bp。其蛋白与黄瓜的同源性最高,属于植物类GSK3/shaggy蛋白激酶家族的第Ⅳ类,与苜蓿(MSK4)蛋白在进化关系上最近,且与拟南芥(AtSK4-1、AtKSK4-2)聚为一枝。保守域结构分析表明,KdSK蛋白具有明显的蛋白激酶的结构域,包括蛋白激酶的ATP结构域和丝氨酸/苏氨酸蛋白激酶活化结构域。实时荧光定量PCR分析表明,该蛋白基因在大叶落地生根的根中表达量最高,且受渗透胁迫(甘露醇)的诱导上调表达。该研究首次从大叶落地生根中克隆出KdSK基因,该研究结果为进一步研究该基因的功能打下了基础。
[Abstract]:Glycogen synthase kinase (SKs) is a serine / threonine protein kinase, which plays an important role in plant organ development and hormone signal transduction, and is involved in biological stress. Response to abiotic stress. The development of plantlets in the rooting of large leaves, with the characteristics of embryogenesis and organogenesis. It is an ideal model for studying asexual reproduction. In order to better understand the molecular mechanism of embryogenic seedling development in the rooting of large leaf, RACE-PCR technique was used in this study. A new gene KdSK was cloned from the rooting of Lymphoma macrophylla. The gene has 423 amino acid residues with molecular weight of 47.79 KD, isoelectric point of 8.37, and its open reading frame length is 1 272 BP. The protein has the highest homology with cucumber. It belongs to the fourth class of plant GSK3/shaggy protein kinases family, and has the closest evolutionary relationship with the alfalfa protein MSK4, and is clustered into one branch with Arabidopsis thaliana AtSK4-1 (AtKSK4-2). The conserved domain analysis shows that the KDSK protein has a distinct protein kinase domain. The ATP domain including protein kinase and serine / threonine protein kinase activated domain. Real-time quantitative PCR analysis showed that the protein gene was the most expressed in rooting roots. The expression of KdSK gene was firstly cloned from the rooting of Lymphoma macrophylla under osmotic stress (mannitol). The results laid a foundation for further study on the function of the gene.
【作者单位】：深圳市日f园林绿化有限公司;暨南大学深圳旅游学院;北京林业大学林学院草坪研究所;华南农业大学林学与风景园林学院广东省草业工程技术研究中心;
【基金】：深圳市科技计划项目(CXZZ20140418110342522) 北京林业大学与厦门日懋城建园林建设股份有限公司产学研合作项目~~
【分类号】：S682.33

【相似文献】