绵羊痘病毒固原株L2R基因的克隆与生物信息学分析

发布时间：2018-03-04 22:25

本文选题：羊痘病毒　切入点：LR基因　出处：《中国畜牧兽医》2017年01期 　论文类型：期刊论文

【摘要】：为分析绵羊痘病毒L2R蛋白的分子特征,本试验提取了绵羊痘病毒固原株(GY)的基因组DNA,设计L2R基因引物,对L2R基因进行扩增,将扩增的基因连接到pGEM-T Easy载体后转化到大肠杆菌Trans 5α感受态细胞,筛选阳性克隆,进行序列测序。利用生物信息学软件对L2R基因序列进行预测分析。结果显示,L2R基因序列含有一个由279个核苷酸组成的开放阅读框,编码92个氨基酸残基组成的多肽,蛋白质分子质量理论值为10.92ku,理论等电点为6.56。该蛋白质二级结构组成分别是α-螺旋占69.57%,β-折叠占9.78%,无规则卷曲占8.70%,延伸链占11.96%。多序列比对分析结果显示,不同羊痘病毒分离株L2R序列高度保守。进化树分析结果显示,GY株与NK、TU及SA株在一个分支,表明它们之间亲缘关系较近。本试验结果为进一步研究L2R蛋白的生物学功能和羊痘病毒早期蛋白的分子相互作用奠定了基础。
[Abstract]:In order to analyze the molecular characteristics of sheep pox virus L2R protein, the genomic DNA of sheep pox virus GY strain was extracted, and the L2R gene primer was designed to amplify the L2R gene. The amplified gene was ligated into pGEM-T Easy vector and transformed into Escherichia coli Trans 5 伪 competent cells to screen positive clones. The sequence was sequenced. The sequence of L2R gene was predicted by bioinformatics software. The results showed that the sequence of L2R gene contained an open reading frame composed of 279 nucleotides and encoded a peptide composed of 92 amino acid residues. The theoretical value of protein molecular mass is 10.92kuand the theoretical isoelectric point is 6.56.The secondary structure of the protein consists of 伪 -helix (69.57), 尾 -fold (9.78), irregular curl (8.70), extended chain (11.96). The results of phylogenetic tree analysis showed that GY strain was in the same branch as NKTU and SA strains. These results provide a basis for the further study of the biological function of L2R protein and the molecular interaction of early protein of sheep pox virus.
【作者单位】：中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室;深圳出入境检验检疫局动植物检验检疫技术中心;
【基金】：国家重点研发计划课题(2016YFD0500907) 甘肃省国际科技合作专项(1604WKCA012);甘肃省国际科技合作专项(1504WKCA055)
【分类号】：S852.654

【相似文献】