与脐带间充质干细胞共培养对乳腺上皮细胞乳脂合成及关键基因表达的影响

发布时间：2018-03-04 23:09

本文选题：脐带间充质干细胞　切入点：乳腺上皮细胞　出处：《畜牧兽医学报》2017年05期 　论文类型：期刊论文

【摘要】：旨在探究奶牛乳腺上皮细胞(BMECs)与脐带间充质干细胞(UC-MSCs)共培养对(BMECs)乳脂合成及关键基因表达的影响。试验共分为8组:共培养组为UC-MSCs和BMECs共培养条件下的不处理组、IGF-1R抑制剂AG1024处理组、Janus激酶和转录活化因子(JAK/STAT)信号通路信号阻断剂AG490处理组及AG1024+AG490处理组,对照组为BMECs单培养条件下的不处理组、IGF-1R抑制剂AG1024组、Janus激酶和转录活化因子(JAK/STAT)信号通路信号阻断剂AG490组及AG1024+AG490处理组。检测各组上清IGF-1、甘油三酯(TG)含量变化;RT-qPCR检测乙酰辅酶A羧化酶(ACACA),脂肪酸合成酶(FASN)和固醇调节元件结合蛋白(Sterol regulatory element binding proteins,SREBP1)基因的相对表达丰度。结果表明,共培养组IGF-1、TG含量均显著高于对照组(P0.05);AG1024处理对IGF-1具有极显著抑制效果(P0.01),显著降低TG含量及ACACA、FASN、SREBP1mRNA相对表达丰度(P0.05);AG490处理对ACACA、FASN、SREBP1mRNA的表达无显著影响(P0.05);AG1024和AG490共同处理较AG1024单独处理各项指标表现差异不显著(P0.05)。综上表明,脐带间充质干细胞能够通过IGF-1促进乳腺上皮细胞乳脂合成及关键基因的表达,JAK2/STAT5信号通路不参与脐带间充质干细胞对乳腺上皮细胞乳脂调控。
[Abstract]:To investigate the effects of co-culture of bovine mammary epithelial cells (BMECs) and umbilical cord mesenchymal stem cells (UC-MSCs) on the synthesis of milk fat and expression of key genes in the milk of BMC cells. The experiment was divided into 8 groups: the co-culture group was untreated under the condition of UC-MSCs and BMECs co-culture. IGF-1R inhibitor AG1024 treatment group, AG490 treatment group and AG1024 AG490 treatment group, The control group was treated with BMECs mono-culture. The level of IGF-1R inhibitor AG1024 was detected by RT-qPCR. The signal pathway blocker AG490 and AG1024 AG490 were used to detect the IGF-1 and triglyceride TGs in the supernatant of each group. The relative expression abundance of acetyl coenzyme A carboxylase, fatty acid synthase (FASN) and sterol regulatory element binding proteins1 (SREBP1) gene was observed. The content of IGF-1G in co-cultured group was significantly higher than that in control group (P0.05AG-1024). The effect of P0.01C on IGF-1 was significantly lower than that of P0.01treatment, and the relative expression abundance of ACA FASNNU SREBP1mRNA and the relative expression of ACA FASNN SREBP1 mRNA were not affected by ACA FASNN SREBP1 mRNA expression in ACA FASNSREBP1 treated with ACA FASNN AG1024 and AG490 alone. The results showed that the expression of ACA FASNSREBP1 mRNA was not affected by ACA FASNN AG1024 and AG490 alone. There was no significant difference in the performance of various indexes between the two groups (P 0.05). Umbilical cord mesenchymal stem cells can promote breast epithelial cell milk fat synthesis and expression of key genes through IGF-1. JAK2 / STAT5 signaling pathway does not participate in the regulation of breast epithelial cell milk fat by umbilical cord mesenchymal stem cells.
【作者单位】：新疆农业大学动物科学学院新疆肉乳用草食动物营养实验室;
【基金】：奶产业体系资助(CARS-37) 国家自然科学基金(31560645) 中国博士后基金新疆维吾尔自治区高等学校科研计划项目(XJEDU2013S17) 2013年度新疆研究生科研创新项目(xjau-2013-yjsky-XJGRI2013113) 新疆肉乳用草食动物营养实验室开放课题
【分类号】：S823

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