棉花SNC2基因的克隆及表达分析

发布时间：2018-03-05 19:18

  本文选题：棉花　切入点：SNC　出处：《分子植物育种》2017年01期 　论文类型：期刊论文

【摘要】：以陆地棉(Gossypium hirsutum)TM-1为材料,利用同源克隆技术从棉花中分离出SNC2基因,以actin基因为内参,采用半定量RT-PCR分析该基因在棉花组织中的表达,并采用实时定量RT-PCR分析不同棉花品种经大丽轮枝菌侵染后,SNC2基因在不同时段、不同组织的表达水平。研究分析发现该基因CDS序列长度为1 308 bp,编码435个氨基酸,蛋白的等电点(p I)为6.98,理论分子量为46.93 k D,命名为SNC2,Gen Bank登录号为AOO35322.1,SNC2基因编码多肽链中包含信号肽和跨膜结构域,且亚细胞定位于质膜,编码受体类蛋白。组织表达分析显示SNC2基因在根、侧根、茎、叶和花中均有表达,茎中的表达量较高,而在苞片组织中基本不表达。该基因在接菌后的感病品种军棉1号的根和耐病品种中棉49号的茎中呈现不断上升的趋势,而在两个棉花品种的其他组织中呈现出"升-降-升"的趋势,且在不同抗性的棉花品种中的表达存在差异,暗示SNC2基因可能在不同抗性品种抵御生物胁迫过程中扮演重要角色,研究结果为进一步研究SNC2基因的功能及应用该基因提供帮助。
[Abstract]:Using Gossypium hirsutum)TM-1 as material, SNC2 gene was isolated from cotton by homologous cloning technique. The expression of SNC2 gene in cotton tissue was analyzed by semi-quantitative RT-PCR using actin as internal reference. Real-time quantitative RT-PCR was used to analyze the expression level of SNC2 gene in different tissues of different cotton varieties infected by Verticillium dahliensis. The length of CDS sequence of this gene was 1 308 BP, encoding 435 amino acids. The isoelectric point I) of the protein is 6.98, and the theoretical molecular weight is 46.93 KD. The accession number of SNC2Gen Bank is AOO35322.1 and SNC2 gene encodes a signal peptide and a transmembrane domain, and the subcells are located in the plasma membrane. The expression of SNC2 gene was found in roots, lateral roots, stems, leaves and flowers. But it was not expressed in bracts. The gene showed an increasing trend in the roots of susceptible variety Junmian 1 and the stem of susceptible variety Zhongmian49 after inoculation. However, in other tissues of the two cotton varieties, there was a tendency of "up-down-rise-rise-rise", and there were differences in expression in different resistant cotton varieties. It is suggested that SNC2 gene may play an important role in the process of resistance to biological stress in different resistant varieties, and the results may be helpful to further study the function of SNC2 gene and its application.
【作者单位】： 新疆师范大学生命科学学院;新疆农业科学院核技术生物技术研究所新疆农作物生物技术重点实验室新疆农业科学院棉花分子机理与分子育种实验室;
【基金】：新疆维吾尔自治区自治区自然基金项目(2013211A037)资助
【分类号】：S435.62;Q943.2
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本文编号：1571537