小麦TaTGW6基因的克隆鉴定和功能标记开发

发布时间：2018-03-05 21:19

  本文选题：TaTGW6　切入点：dCAPS　出处：《中国农业科学院》2016年博士论文　论文类型：学位论文

【摘要】：TGW6编码一个新型吲哚乙酸葡萄糖水解酶,对水稻千粒重和产量都有显著影响。通过同源克隆分离了来自于小麦同源染色体群2L、3L和7S的9个TGW6同源基因。它们都没有内含子。为了检测这些基因的多态性,两组千粒重显著不同的8个小麦品系用作分子标记开发的初始材料,其中高千粒重品种包括豆麦、PH85-1-1、鄂86642和临旱6114；低千粒重品种包括洋小麦、豫麦21、山东955159和陕898-33。通过克隆测序获得了这8个品系的所用9个TGW同源基因,其中只有TaTGW6-A1l有三个单核苷酸多态性(SNP)位点显著与千粒重关联,并且这三个SNP形成两个单倍型：TaTGW6-A1a和TaTGW6-A1b。根据这两个单倍型,开发了一个衍生酶切扩增多态性序列(dCAPS)分子标记TaTGW6-A1-CAPS。利用中国春/周8425B的近等基因系(RIL)作图群体并结合小麦90K iSelect SNP芯片进行连锁分析,TaTGW6-A1-CAPS被进一步定位在小麦3A染色体长臂的SNP标记BobWhite_c47304_56和GENE_3365_61之间,并且TaTGW6-Al-CAPS和BobWhite_c47304_56之间检测到了一个控制产量的QTL。在四个环境下这个QTL可以解释平均17.4%的表型变异。利用不同小麦生态区的242个小麦品系进行关联分析,结果显示TaTGW6-A1和千粒重显著相关。进一步分析表明约80%小麦品种具有高千粒重的单倍型TaTGW6-A1a,说明在育种实践中对TaTGW6的高千粒重单倍型已经进行了正选择。总之,小麦TGW6同源基因的分离和相应分子标记的开发为小麦高产育种提供了很有利用价值的基因信息和工具。
[Abstract]:TGW6 encodes a novel indoleacetic acid glucose hydrolase. Nine TGW6 homologous genes from wheat homologous chromosome group 2Ln3L and 7s were isolated by homologous cloning. None of them had introns. In order to detect the polymorphism of these genes, Eight wheat lines with significantly different 1000-grain weights were used as the initial materials for molecular marker development, among which the high 1000-grain weight varieties included Ph85-1-1, E 86642 and Linhan 6114, and the low 1000-grain weight varieties included Yang wheat. Yumai 21, Shandong 955159 and Shan 898-33.Nine TGW homologous genes were obtained by cloning and sequencing. Among them, only three single nucleotide polymorphisms (SNPs) were found in Yumai 21, Shandong 955159 and Shan898-33. among them, only three single nucleotide polymorphisms (SNPs) were significantly associated with 1000-grain weight. And the three SNP forms two haplotypes: TaTGW6-A1a and TaTGW6-A1b. according to these haplotypes, A molecular marker TaTGW6-A1-CAPS. using Chinese Spring / Zhou8425B near isogenic line rill was developed and linked to wheat 90K iSelect SNP microarray. TaTGW6-A1-CAPS was further located in wheat 3A staining. The SNP of the long arm marked between BobWhite_c47304_56 and GENE_3365_61. And a QTL was detected between TaTGW6-Al-CAPS and BobWhite_c47304_56, which could explain an average of 17.4% phenotypic variations in four environments, using 242 wheat lines in different wheat ecological regions for correlation analysis. The results showed that there was a significant correlation between TaTGW6-A1 and 1000-grain weight. Further analysis showed that about 80% wheat varieties had high 1000-grain weight haplotype TaTGW6-A1a, which indicated that the high 1000-grain weight haplotype of TaTGW6 had been selected in breeding practice. The isolation of wheat TGW6 homologous genes and the development of corresponding molecular markers provide valuable genetic information and tools for wheat breeding with high yield.
【学位授予单位】：中国农业科学院
【学位级别】：博士
【学位授予年份】：2016
【分类号】：S512.1
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本文编号：1571949