禽腺病毒4型河南株Hexon全基因的克隆及遗传进化分析

发布时间：2018-03-08 00:06

本文选题：禽心包积水-包涵体肝炎综合征　切入点：禽腺病毒型　出处：《西北农林科技大学学报(自然科学版)》2017年06期 　论文类型：期刊论文

【摘要】：【目的】分析禽心包积水-包涵体肝炎综合征的主要病原禽腺病毒4型(FAV-4)在河南省的流行和遗传变异情况。【方法】对2015年8-10月送检的40份疑似FAV-4感染的病料进行PCR阳性检测,同时根据采集地区与宿主的差异性,选取7份FAV-4阳性样品进行了Hexon全基因的扩增、克隆、测序和遗传进化分析。【结果】通过扩增Hexon基因421bp的片段,成功建立了禽腺病毒4型的PCR检测方法;40份家禽肝脏样品中,PCR检测显示35份为FAV-4阳性,阳性率为87.5%;成功扩增了包含Hexon基因全序列的片段,长度为2 889bp;7株FAV-4河南株的Hexon基因与GenBank中的12株FAV-4参考毒株Hexon基因序列之间核苷酸序列同源性为98.5%~99.8%,与参考株相比存在碱基的插入、突变现象;FAV-4河南株Hexon全基因推导的氨基酸序列与参考株之间的同源性为98.2%~99.8%,且发生变化的位置集中在前段和中段。【结论】FAV-4河南株与印度分离株亲缘关系较近,但与韩国分离株亲缘关系相对较远。
[Abstract]:[objective] to analyze the prevalence and genetic variation of avian adenovirus type 4 (FAV-4) in avian pericardial hydronephrosis and inclusion body hepatitis syndrome. [methods] 40 samples of suspected FAV-4 infection from 2015 to October were tested for PCR positive. At the same time, according to the difference between the region and the host, 7 FAV-4 positive samples were selected for Hexon gene amplification, cloning, sequencing and genetic evolution analysis. [results] by amplifying the 421bp fragment of Hexon gene, A PCR method for detection of avian adenovirus type 4 was successfully established. In 40 samples of poultry liver, 35 samples were positive for FAV-4, the positive rate was 87.5, and the fragment containing the whole sequence of Hexon gene was amplified successfully. The nucleotide sequence homology between the Hexon gene of the Henan strain of FAV-4 and the Hexon gene of the 12 FAV-4 reference strains in GenBank was 98.5 ~ 99.8, and the nucleotide sequence of the Hexon gene of the Henan strain was 98.5 ~ 99.8, compared with the reference strain, the nucleotide sequence was inserted. The amino acid sequence deduced from the whole Hexon gene of FAV-4 Henan strain was 98.2% and 99.8% of the reference strain, and the location of the change was concentrated in the front and middle segments. [conclusion] the relationship between FAV-4 Henan strain and Indian isolate is close, and the amino acid sequence of FAV-4 strain is 98.2% and 99.8% respectively. [conclusion] there is a close relationship between FAV-4 Henan strain and Indian isolate. But the relationship with Korean isolate is relatively distant.
【作者单位】：河南农业大学牧医工程学院;郑州市猪重大疫病防控重点实验室;
【基金】：河南省重大科技专项(111100110300) 河南省产学研项目(132107000002)
【分类号】：S852.65

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