Hes1基因沉默可抑制食管腺癌OE33细胞的增殖、迁移及侵袭

发布时间：2018-03-08 22:23

本文选题：食管肿瘤　切入点：基因沉默　出处：《肿瘤》2016年07期 　论文类型：期刊论文

【摘要】：目的 :探讨发状分裂相关增强子1(hairy and enhancer of split 1,Hes1)基因表达对食管腺癌OE33细胞增殖、侵袭及迁移的影响。方法 :应用实时荧光定量PCR及蛋白质印迹法检测人食管黏膜上皮HEEC细胞、食管鳞癌TE-1和食管腺癌OE33细胞中Hes1基因的表达水平。将特异性针对Hes1基因的sh RNA重组慢病毒感染食管腺癌OE33细胞,然后应用实时荧光定量PCR及蛋白质印迹法检测Hes1基因的沉默效率,并通过CCK-8、划痕愈合实验以及Transwell迁移和侵袭实验分别检测Hes1基因沉默对OE33细胞增殖、迁移及侵袭能力的影响。结果 :与HEEC和TE-1细胞相比,OE33细胞中Hes1 m RNA和蛋白的表达水平明显较高(P值均0.01)。Hes1-sh RNA重组慢病毒感染OE33细胞后,Hes1 m RNA和蛋白表达水平均明显下调(P值均0.01),并且细胞的增殖、迁移及侵袭能力均明显降低(P值均0.01)。结论 :沉默Hes1基因的表达能一定程度上抑制OE33细胞的增殖、迁移及侵袭,提示Hes1可能是一个潜在的食管腺癌治疗靶点。
[Abstract]:Objective: to investigate the effect of hairy and enhancer of split 1 Hes1 gene expression on proliferation of esophageal adenocarcinoma OE33 cells. Methods: Real-time quantitative PCR and Western blotting were used to detect human esophageal epithelial HEEC cells. Expression of Hes1 gene in esophageal squamous cell carcinoma (TE-1) and esophageal adenocarcinoma (OE33) cells. Then real-time fluorescence quantitative PCR and Western blotting were used to detect the silencing efficiency of Hes1 gene. CCK-8, scratch healing test and Transwell migration and invasion assay were used to detect the proliferation of OE33 cells by Hes1 gene silencing. Results: compared with HEEC and TE-1 cells, the expression level of Hes1 m RNA and protein was significantly higher (P = 0.01). Hes1-sh RNA recombinant lentivirus infected OE33 cells with Hes1 m RNA and protein. The P value was 0.01g, and the proliferation of the cells was observed. Conclusion: silencing the expression of Hes1 gene can inhibit the proliferation, migration and invasion of OE33 cells to some extent, suggesting that Hes1 may be a potential therapeutic target for esophageal adenocarcinoma.
【作者单位】：复旦大学附属浦东医院肿瘤内科;湖北医药学院基础医学院;
【基金】：上海市科学技术委员会科研计划项目(编号:13ZR1437300) 上海市浦东新区卫生系统学科带头人培养计划项目(编号:PWRd2012-15)~~
【分类号】：R735.1

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