猴头菇多糖对氧化应激状态下猪小肠上皮细胞紧密连接相关基因表达的影响

发布时间：2018-03-08 23:11

本文选题：猴头菇多糖　切入点：猪小肠上皮细胞　出处：《中国兽医科学》2017年01期 　论文类型：期刊论文

【摘要】：为考察猴头菇多糖(HEP)对氧化应激状态下猪小肠上皮细胞(IPEC-J2)的增殖和紧密连接(TJ)相关基因表达的影响,通过100 g/m L脂多糖(LPS)处理建立IPEC-J2细胞氧化应激模型,在预试验基础上选用125、250、500 g/m L不同质量浓度的HEP处理细胞2、4、8 h,用四甲基偶氮唑盐(MTT)法检测其对正常及氧化应激的IPEC-J2细胞增殖的影响;选取其中最佳的HEP浓度,依上述方法处理细胞,实时荧光定量PCR法检测TJ相关的Occludin、Claudin及ZO-1基因m RNA表达的变化情况。结果显示,不同的HEP浓度和作用时间对正常细胞增殖均有显著影响(P0.05),对LPS诱导的细胞氧化应激均有缓解作用(P0.05);HEP的最佳质量浓度为250 g/m L、最佳作用时间为4 h;250 g/m L的HEP作用4、8 h均能显著提高氧化应激状态下,IPEC-J2细胞Occludin、Claudin和ZO-1基因的m RNA表达量(P0.05)。结果表明,HEP能够促进氧化应激状态下IPEC-J2细胞TJ相关基因的表达,缓解LPS诱导的氧化应激对IPEC-J2屏障功能的损伤。
[Abstract]:In order to investigate the effect of hericium Erinaceus polysaccharides (HEP) on the proliferation of IPEC-J22 in porcine intestinal epithelial cells under oxidative stress and the expression of genes related to tight ligand (TJ), the oxidative stress model of IPEC-J2 cells was established by 100g / mL lipopolysaccharide (LPS) treatment. On the basis of pre-test, 125,250,500 g / mL HEP was used to treat the cells for 8 h. The effects of HEP on the proliferation of normal and oxidative stress IPEC-J2 cells were detected by tetramethylazolium triazolium (MTT) method, and the optimal HEP concentration was selected. The expression of TJ related Occludinia Claudin and ZO-1 gene m RNA was detected by real-time fluorescence quantitative PCR. The effects of HEP concentration and time on the proliferation of normal cells were significant (P 0.05). The best mass concentration of P0.05 Hep was 250g / mL, and the best time was 4 h (250g / mL) of HEP. H significantly increased the expression of m RNA of Occludinia Claudin and ZO-1 genes in IPEC-J2 cells under oxidative stress. The results showed that Hep could promote the expression of TJ-related genes in IPEC-J2 cells under oxidative stress. To alleviate the damage of IPEC-J2 barrier induced by oxidative stress induced by LPS.
【作者单位】：福建农林大学福建省兽医中药与动物保健重点实验室;
【基金】：国家自然科学基金项目(31502110)
【分类号】：S853.74

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