母系印迹表达基因3在结直肠癌组织中表达及其与血管生成的关系

发布时间：2018-03-09 04:31

本文选题：结直肠肿瘤　切入点：RNA　出处：《中国普通外科杂志》2016年10期 　论文类型：期刊论文

【摘要】：目的:探讨结直肠癌组织中长链非编码RNA母系印迹表达基因3(MEG3)的表达及其与肿瘤血管生成的关系。方法:用RT-PCR法检测42例结直肠癌患者癌组织与对应的癌旁组织标本,以及人结肠癌SW48细胞与人正常结肠NCM460细胞中MEG3表达,分析MEG3表达与患者临床病理因素的关系;免疫组化法检测上述组织标本中血管内皮生长因子(VEGF)的表达及血管密度,分析VEGF表达、血管密度与MEG3表达的相关性。结果:MEG3相对表达量在结直肠癌组织明显低于癌旁组织(0.12 vs.1.00,P=0.003),在SW48细胞中明显低于NCM460细胞(0.15 vs.1.00,P=0.007);MEG3的相对表达量与患者肿瘤位置、浸润深度、分化程度和淋巴结转移无明显关系(均P0.05)。结直肠癌组织中,VEGF表达与血管密度均明显高于癌旁组织(0.13 vs.0.09;50.34 vs.36.57,均P0.05);MEG3表达水平和VEGF表达水平及血管密度均呈负相关(r=-0.304、-0.342,均P0.05)。VEGF是MEG3表达水平的独立影响因素(P=0.005)。结论:结直肠癌组织中MEG3表达降低,从而可能促进肿瘤组织VEGF表达量与血管生成。
[Abstract]:Objective: to investigate the expression of long chain noncoding RNA motif imprinted gene 3mEG3 and its relationship with tumor angiogenesis in colorectal carcinoma. Methods: 42 colorectal cancer tissues and corresponding paracancerous tissues were detected by RT-PCR method. The expression of MEG3 in human colon cancer SW48 cells and normal colon NCM460 cells was analyzed, and the relationship between the expression of MEG3 and the clinicopathological factors of the patients was analyzed, the expression of vascular endothelial growth factor (VEGF) and vascular density were detected by immunohistochemistry. Results the relative expression of VEGF, vascular density and MEG3 expression in colorectal cancer tissues was significantly lower than that in adjacent tissues of colorectal cancer 0.12 vs.1.00 P0. 003, and in SW48 cells was significantly lower than that in NCM460 cells (0. 15 vs.1. 00 P0. 007) and the tumor location, depth of invasion, depth of invasion, and the relative expression of MEG3 was significantly lower in SW48 cells than that in NCM460 cells (0. 15 vs.1. 00 P0. 007, P 0. 007). There was no significant correlation between the degree of differentiation and lymph node metastasis (all P 0.05). The expression of VEGF and vascular density in colorectal carcinoma were significantly higher than those in adjacent tissues (0.13 vs.0.09 ~ 50.34 vs.36.57). There was a negative correlation between the expression level of MEG3 and the expression of VEGF and vascular density in colorectal cancer tissues. Conclusion: the expression of MEG3 is decreased in colorectal carcinoma. This may promote the expression of VEGF and angiogenesis in tumor tissues.
【作者单位】：吉林省肿瘤医院内三科;吉林省肿瘤医院病理科;吉林省肿瘤医院放疗三科;
【分类号】：R735.34

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