硬化蛋白基因在淇河鲫成鱼不同肌间骨相邻肌组织的表达差异分析

发布时间：2018-03-09 07:15

本文选题：淇河鲫　切入点：肌间骨　出处：《中国生物化学与分子生物学报》2016年12期 　论文类型：期刊论文

【摘要】：为探究硬化蛋白(sclerostin,SOST)基因在淇河鲫肌间骨不同分布位置中的作用,以淇河鲫成鱼为研究对象,对其肌间骨的形态与分布进行了统计,并在此基础上利用qRT-PCR技术检测SOST基因在淇河鲫背部和尾部肌肉中的mRNA表达,通过Western印迹和免疫组织化学技术检测SOST蛋白的表达定位情况。结果显示:淇河鲫肌间骨包括髓弓小骨和脉弓小骨,髓弓小骨为56~58根,位于淇河鲫背部肌肉的肌隔中,脉弓小骨27~28根,分布于泄殖孔之后的肌隔中。背部肌肉SOST的mRNA表达水平显著高于尾部肌肉(P0.05)。与mRNA表达水平相比,背部肌肉和尾部肌肉中SOST蛋白呈现相同表达趋势。免疫组织化学结果显示SOST在肌隔组织中特异性表达,并且在背部肌肉中的表达强烈,尾部肌肉中没有阳性反应。上述结果表明,SOST在淇河鲫肌间骨的背部和尾部肌肉中存在差异性表达,从而影响肌隔组织中肌间骨的骨化,对背部和尾部肌间骨形态发生产生影响。
[Abstract]:In order to investigate the role of sclerosing protein sclerotin (SOST) gene in different distribution positions of muscle bone in Qihe crucian carp, the morphology and distribution of muscle bone in adult crucian carp were analyzed. The expression of SOST gene in the back and tail muscles of Qihe crucian carp was detected by qRT-PCR technique. The expression and localization of SOST protein were detected by Western blotting and immunohistochemistry. The results showed that the intramuscular bone of Qihe crucian carp included medullary arch bone and pulse-arch bone, and the medullary arch bone was 56 ~ 58, which was located in the muscle septum of dorsal muscle of Qihe crucian carp. The expression of SOST in the dorsal muscle was significantly higher than that in the tail muscle (P 0.05). Compared with the mRNA expression level, the expression of mRNA in the dorsal muscle was significantly higher than that in the caudal muscle. The results of immunohistochemistry showed that SOST was expressed specifically in septum and strongly in back muscle. There was no positive reaction in the tail muscles. The results indicated that there were differences in the expression of Sost in the back and tail muscles of crucian carp, which affected the ossification of the intermuscular bone in the septum and the morphogenesis of the dorsal and caudal muscles.
【作者单位】：河南师范大学水产学院;
【基金】：国家自然科学基金(No.31402294) 河南省教育厅科学技术研究重点项目(No.14B24005) 河南师范大学博士科研启动经费(No.qd13056)资助~~
【分类号】：Q953

【参考文献】