结直肠癌组织KRAS、BRAF基因突变情况及其临床意义

发布时间：2018-03-10 21:22

本文选题：结直肠癌　切入点：KRAS基因　出处：《山东医药》2017年16期 　论文类型：期刊论文

【摘要】：目的探讨结直肠癌组织KRAS、BRAF基因突变情况及其临床意义。方法选择行手术治疗的结直肠癌患者262例,采用ARMS-PCR法检测其结直肠癌组织KRAS基因外显子2第12、13位密码子及BRAF V600E基因突变情况,分析其与患者临床病理参数、肝转移及预后的关系。结果 262例结肠癌患者中女性患者98例。262例份结肠癌组织中,KRAS基因突变49例(18.7%),其中第12位密码子突变37例(包括1例KRAS复合突变),第13位密码子突变12例;184例份结肠癌组织中,BRAF V600E基因突变6例。结直肠癌组织KRAS基因突变与患者性别(女性)、原发肿瘤直径、远处转移情况均有关,BRAF基因突变与民族、家族史、原发肿瘤分化程度均有关(P均0.05)。同时性肝转移患者KRAS基因突变率高于异时性肝转移患者,KRAS基因G13D突变率低于异时性肝转移患者(P均0.05)。KRAS基因突变的结直肠癌患者生存时间短于未突变者(P0.05)。KRAS基因突变是结直肠癌患者预后的独立危险因素(P0.01)。BRAF基因突变与未突变者生存时间比较差异无统计学意义(P0.05)。结论结直肠癌组织KRAS基因突变率高,BRAF基因突变率低;KRAS基因突变多发生于女性,与结直肠癌的发生、发展有关。
[Abstract]:Objective to investigate the mutation of KRAS-BRAF gene in colorectal cancer tissues and its clinical significance. Methods 262 patients with colorectal cancer were selected for surgical treatment. ARMS-PCR method was used to detect the mutation of KRAS gene exon 2 at codon 12 and BRAF V600E gene, and to analyze the relationship between the mutation and the clinicopathological parameters of colorectal cancer. Results among 262 cases of colon cancer, 98 cases were female, 49 cases were mutation of KRAS gene, 37 cases were codon 12 mutation (including 1 case of KRAS complex mutation, 13 case was 13). Codon mutation was found in 12 patients with colonic cancer and 6 patients with BRAF V600E gene mutation. The mutation of KRAS gene was associated with the sex of the patient (female, primary tumor diameter). Distant metastasis was related to mutation of BRAF gene and nationality and family history. The mutation rate of KRAS gene in patients with simultaneous liver metastasis was higher than that in patients with heterotopic liver metastasis. The G13D mutation rate of KRAS gene was lower than that of patients with heterotopic liver metastasis. The mutation of P0.05N. KRAS gene is an independent risk factor for the prognosis of colorectal cancer patients. There is no significant difference in survival time between the patients with P0.01U. BRAF gene mutation and that without mutation. Conclusion the mutation rate of KRAS gene in colorectal cancer tissue is high. The low mutation rate of BRAF gene and KRAS gene mutation occurred mostly in women. It is related to the occurrence and development of colorectal cancer.
【作者单位】：广西医科大学附属肿瘤医院;
【基金】：广西科学研究与技术开发计划基金资助项目(桂科攻1298003-2-8) 南宁市青秀区科学研究与技术开发计划基金资助项目(2015S03)
【分类号】：R735.34

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