长丰鲫c型溶菌酶基因的克隆及其在细菌感染条件下的表达分析

发布时间：2018-03-11 14:13

本文选题：长丰鲫(Chang　切入点：Feng　出处：《淡水渔业》2017年03期 　论文类型：期刊论文

【摘要】：为了解c型溶菌酶基因与长丰鲫(Chang Feng Carassius auratus)的抗菌效应关系,本研究利用同源克隆方法获得长丰鲫c型溶菌酶基因cDNA全长序列。结果显示:c型溶菌酶基因全长698 bp,包括5'端非翻译区60 bp,3'端非翻译区200 bp,开放阅读框438 bp,编码145个氨基酸。长丰鲫c型溶菌酶基因在肾脏组织中表达量最大,在脾脏、肠道、心脏和脑中大量表达,在肝脏和鳃中表达量相对较低,在皮肤和肌肉中几乎不表达。长丰鲫在感染迟钝爱德华氏菌、嗜水气单胞菌和金黄色葡萄球菌后,在肝脏、肾脏、脾脏和鳃等组织中基因表达量均发生显著变化,出现不同程度的上调。感染迟钝爱德华氏菌后,在脾脏中的上调幅度最大,其次为鳃、肝脏和肾脏;感染嗜水气单胞菌后,在脾脏中上调程度最大,其次是鳃;感染金黄色葡萄球菌后,在脾脏中上调幅度最大。在肝脏和肾脏中,经金黄色葡萄球菌刺激后c型溶菌酶基因的上调幅度最高;在脾脏和鳃中,经嗜水气单胞菌感染后c型溶菌酶基因上调幅度最高。三种刺激后,c型溶菌酶基因升高幅度的不同,说明不同刺激使鱼体组织产生的应激反应能力不同。
[Abstract]:To study the relationship between c type lysozyme gene and Chang Feng Carassius auratus. In this study, the full-length cDNA sequence of c type lysozyme gene of Changfeng crucian carp was obtained by homologous cloning method. The results showed that the full length of lysozyme gene of type C was 698 BP, including the untranslated region of 60 BP at the 5'end and the untranslated region at the end of 3', and the open reading frame was 438bp. 145 amino acids. The c lysozyme gene of Changfeng crucian carp was the most expressed in kidney. It is expressed in spleen, intestine, heart and brain, but is relatively low in liver and Gill, but almost not in skin and muscle. After infection with Edwardiella dullus, Aeromonas hydrophila and Staphylococcus aureus, In the liver, kidney, spleen and Gill, the expression of genes changed significantly, and there were different degrees of upregulation. After infection with Edwardsiella dullus, the upregulation was the largest in the spleen, followed by the Gill, liver and kidney. After infection with Aeromonas hydrophila, the degree of upregulation was the highest in the spleen, followed by the Gill. After infection with Staphylococcus aureus, the upregulation was the largest in the spleen. After stimulation by Staphylococcus aureus, the up-regulation of c type lysozyme gene was the highest, while in the spleen and Gill, the up-regulation of c type lysozyme gene was the highest after infection by Aeromonas hydrophila. The results showed that the stress response ability of fish tissues was different with different stimuli.
【作者单位】：华中农业大学水产学院;中国水产科学研究院长江水产研究所;
【基金】：“十二五”国家科技支撑计划项目(2012BAD26B02)资助~~
【分类号】：S943

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