马铃薯StNCED1基因过表达载体的构建及其遗传转化

发布时间：2018-03-12 12:08

本文选题：NCED基因　切入点：植物表达载体　出处：《分子植物育种》2017年01期 　论文类型：期刊论文

【摘要】：马铃薯块茎的休眠和发芽对于块茎生产和加工极为重要,延长块茎休眠期有助于降低块茎水分和养分的消耗,从而提高其应用价值。本研究用PCR方法从马铃薯中克隆了9-顺式-环氧类胡萝卜素双氧合酶(NCED)编码基因St NCED1,并构建了马铃薯块茎特异表达启动子CIPP驱动的St NCED1基因的植物过表达载体p BIC-St NCED1,然后通过根癌农杆菌介导法转化马铃薯栽培品种"陇薯3号"获得转化植株,经卡那霉素抗性筛选得到5株抗性苗,PCR检测证明St NCED1基因已整合到马铃薯的基因组中。qRT-PCR检测表明St NCED1基因在转基因植株试管薯中的表达量分别比未转基因的对照增加2.10~8.09倍。该研究有助于培育块茎休眠期延长的马铃薯新种质。
[Abstract]:The dormancy and germination of potato tubers are very important for tuber production and processing. Prolonging the dormancy period of tubers is helpful to reduce the consumption of water and nutrients in tubers. In order to improve its application value, we cloned 9-cis-epoxy-carotenoid dioxygenase (NCED1) encoding gene St NCED1 from potato by PCR method, and constructed St NCED1 gene driven by potato tuber specific expression promoter CIPP. The plant overexpression vector p BIC-St NCED1 was transformed into potato cultivar "Longshu 3" by Agrobacterium tumefaciens mediated by Agrobacterium tumefaciens. Five resistant seedlings were screened by kanamycin resistance. The results showed that St NCED1 gene had been integrated into potato genome. QRT-PCR analysis showed that St NCED1 gene expression in transgenic tuber was higher than that in untransgenic control. This study was helpful to cultivate new potato germplasm with prolonged dormancy of tuber.
【作者单位】：甘肃省作物遗传改良与种质创新重点实验室甘肃省干旱生境作物学省部共建国家重点实验室培育基地;甘肃农业大学生命科学技术学院;
【基金】：国家自然科学基金(31160298;31560413) 甘肃省杰出青年科学基金项目(1308RJDA011) 甘肃农业大学“伏羲人才”计划项目(FXRC20130102)共同资助
【分类号】：S532;Q943.2

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