miR-210的红细胞系相关下游靶基因预测

发布时间：2018-03-12 17:42

本文选题：微小RNA　切入点：miR-　出处：《山东医药》2017年03期 　论文类型：期刊论文

【摘要】：目的利用生物信息学方法预测miR-210的红细胞系(以下简称红系)相关下游靶基因。方法利用NCBI比对分析miR-210成熟序列在各哺乳动物之间的保守性。运用基因数据库筛选miR-210的下游靶基因。采用Cytoscape中的BINGO插件对预测的miR-210下游靶基因进行GO分类富集分析。筛选参与红系增殖、分化、凋亡的miR-210下游靶基因,采用DAVID和KEGG数据库进行信号转导通路富集分析后,预测miR-210的红系相关下游靶基因。利用Targetscan在线工具验证靶基因与miR-210的相互作用关系。结果 miR-210成熟序列在多物种间具有高度保守性。筛选出416个与miR-210相关性较高的预测靶基因,其分子功能集中在蛋白结合、离子跨膜运输活动、酶结合等,参与的生物学过程集中在细胞信号转导、突触传导等,参与构成的细胞组分主要有突触小泡、细胞质、细胞器等。筛选出26个与红系增殖、分化、凋亡密切相关的miR-210下游靶基因,参与调控的信号通路主要集中于癌症信号通路、PI3K-Akt信号通路等。初步确定Smad3、Mapk10、Stat5a、Ywhag、Ctgf、Kitl6基因与红系增殖、分化密切相关,其中Smad3、Mapk10、Stat5a、Ywhag、Kitl可能受miR-210的直接调控,Ctgf可能受miR-210的间接调控。结论初步筛选出miR-210的红系相关下游靶基因Smad3、Mapk10、Stat5a、Ywhag、Ctgf、Kitl6,这些靶基因可能与红系的增殖、分化有关。
[Abstract]:Objective to predict the downstream target genes associated with erythrocyte lineage (red line) of miR-210 by bioinformatics. Methods NCBI alignment was used to analyze the conservatism of miR-210 mature sequences among mammals. The downstream target genes of miR-210 were selected. The predicted downstream target genes of miR-210 were classified and enriched by BINGO plug-in in Cytoscape. The downstream target genes of miR-210, which were differentiated and apoptotic, were analyzed by DAVID and KEGG database after enrichment of signal transduction pathway. Targetscan online tools were used to verify the interaction between target genes and miR-210. Results miR-210 mature sequences were highly conserved among multiple species. 416 predictive target genes with high correlation with miR-210 were screened. Its molecular functions are concentrated in protein binding, ion transmembrane transport, enzyme binding, etc. The biological processes involved in the biological processes are cell signal transduction, synaptic transmission, etc. The cellular components involved in the formation are mainly synaptic vesicles, cytoplasm, etc. Cell organelles and so on. 26 downstream target genes of miR-210 were screened, which were closely related to the proliferation, differentiation and apoptosis of erythrocytes. The signal pathways involved in the regulation were mainly focused on the PI3K-Akt signaling pathway of cancer. Smad3mapk10Stat5aHYwhaga Kitl may be directly regulated by miR-210 and CTGF may be indirectly regulated by miR-210. Conclusion the downstream target gene Smad3mapk10Stat5aCtgfKitl6 of miR-210 may be related to the proliferation and differentiation of red lines.
【作者单位】：青海大学医学院;
【基金】：国家自然科学基金资助项目(81441116) 青海省科技厅(应用)基础研究计划项目(2012-Z-729) 青海大学“123高层次人才培养工程”中青年学术带头人基金;青海大学医学院中青年科研基金团队项目(2014-KT-1);青海大学医学院大学生科技创新基金项目
【分类号】：Q78

【相似文献】