番茄GRAS基因家族生物信息学分析及部分抗性相关基因鉴定分析

发布时间：2018-03-12 17:48

本文选题：番茄　切入点：GRAS转录因子　出处：《东北农业大学》2017年硕士论文　论文类型：学位论文

【摘要】：GRAS转录因子是植物中特有的,已有研究表明GRAS转录因子参与包括草本植物、木本植物、水生植物在内的多种植物的复杂生物学过程,尤其与植物逆境胁迫应答调控关系密切,该基因家族的研究目前多集中在模式植物拟南芥中,在番茄中的相关研究较少。本研究利用生物信息学方法和软件,对番茄基因组中GRAS家族成员进行全面鉴定挖掘,并对该家族进行了系统的生物信息学分析,同时对部分家族成员进行了组织特异性及逆境胁迫下的表达模式分析,为该家族基因的深入研究奠定基础。研究结果表明:1.番茄基因组共编码53条GRAS转录子基因,并且被定位到12条染色体上,系统进化分析得到GRAS基因家族可被划分为八个亚族,分别为Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ、Ⅵ、Ⅶ、Ⅷ,染色体定位分析显示GRAS基因在12条染色体中呈不均匀分布。且并不是所有的GRAS基因家族的蛋白序列都是高度保守的,保守元件分析表明部分番茄GRAS基因家族成员是高度保守的。2.对部分典型的GRAS基因进行组织特异性表达模式分析,结果表明,这些基因在番茄的不同组织器官中均有表达,但总体上,根中相对表达量较高,茎和果中相对表达量较低。3.在逆境胁迫应答分析中所选的12条基因在不同的逆境胁迫诱导下,表达量均有不同程度的增加,其中GRAS家族成员SLGRAS43的表达量提高最为显著。4.成功构建了p TRV-SLGRAS43沉默载体并转化至番茄植株体内,获得SLGRAS43基因沉默植株,对基因沉默植株进行抗性相关生理指标检测,结果表明:SLGRAS43参与番茄幼苗的抗旱和抗冷生理过程。
[Abstract]:GRAS transcription factors are endemic to plants. It has been shown that GRAS transcription factors are involved in the complex biological processes of many plants, including herbaceous plants, woody plants and aquatic plants, especially in the regulation of plant stress response. Most of the studies on this gene family are concentrated in Arabidopsis thaliana, but not in tomato. In this study, bioinformatics methods and software were used to identify and excavate the members of GRAS family in tomato genome. The family was systematically analyzed by bioinformatics, and some members of the family were analyzed for tissue specificity and expression patterns under stress. The results show that the tomato genome encodes 53 GRAS transcriptional genes and is located on 12 chromosomes. Phylogenetic analysis shows that the GRAS gene family can be divided into eight subfamilies. Chromosomal localization analysis showed that GRAS gene was unevenly distributed in 12 chromosomes, and not all the protein sequences of GRAS gene family were highly conserved. Conservative element analysis showed that some members of tomato GRAS gene family were highly conserved. 2. Tissue specific expression patterns of some typical GRAS genes were analyzed. The results showed that these genes were expressed in different tissues and organs of tomato. But in general, the relative expression amount in root was higher, and the relative expression in stem and fruit was lower. 3. The 12 genes selected in the stress response analysis had different degrees of increase under different stress stress induction. Among them, the expression of SLGRAS43 in GRAS family was increased most significantly. 4. The expression of p TRV-SLGRAS43 silencing vector was successfully constructed and transformed into tomato plants. The SLGRAS43 gene silencing plants were obtained, and the physiological indexes related to the resistance of SLGRAS43 gene silencing plants were detected. The results showed that: SLGRAS43 was involved in the physiological process of drought and cold resistance of tomato seedlings.
【学位授予单位】：东北农业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S641.2

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