MTMR3基因多态性与非吸烟者肺癌易感性

发布时间：2018-03-13 12:30

  本文选题：肺癌　切入点：非吸烟者　出处：《中华肿瘤防治杂志》2017年02期 　论文类型：期刊论文

【摘要】：目的位于染色体22q12.2区域的MTMR3(myotubularin related protein 3)基因是调控肌维管束蛋白表达的基因,MTMR3基因过度表达与肿瘤等疾病发生有关。本研究旨结合表达数量性状(expression Quati tatire Trait Loci,eQTL)信息探讨MTMR3基因多态性与非吸烟者肺癌易感性的关系,为探究肺癌的发病机制提供依据。方法对肺癌易感区域22q12.2进行连锁不平衡和eQTL分析,筛选具有调控基因表达的致病位点并预测其调控的基因。本研究采用病例对照研究方法,病例为2013-03-05-2014-12-30辽宁省肿瘤医院(96例)、中国医科大学附属第一医院(92例)、中国医科大学附属第四医院(90例)、沈阳军区总医院(95例)和人民解放军沈阳二0二医院(88例)5所三甲级医院的原发性肺癌患者461例(病例组),同期社区中健康对照472名(对照组)。应用TaqMan基因分型技术对rs36605位点进行基因分型。采用t检验比较年龄在病例组与对照组间分布的差异,采用χ~2检验比较性别、各基因型以及各环境暴露因素在病例组与对照组间分布的差异,应用Logistic回归计算OR值及其95%置信区间(CI)。结果经eQTL分析得到rs36605位点是MTMR3基因的一个顺式eQTL,rs36605位点可能与MTMR3基因的表达有关。以TT基因型为参照,AT基因型(OR=0.81,95%CI为0.60~1.10,P=0.178)和AA基因型(OR=1.85,95%CI为0.61~5.59,P=0.276)与肺癌的患病风险无统计学关联。也未发现在显性模型(OR=0.85,95%CI为0.63~1.15,P=0.291)以及隐形模型(OR=1.94,95%CI为0.64~5.86,P=0.238)中存在此关联。试验验证了烹饪油烟暴露增加了肺癌的患病风险,调整OR=1.61,95%CI为1.06~2.45,P=0.025,但未发现烹饪油烟暴露与rs36605位点多态性之间存在交互作用,P0.05。结论试验未发现,22q12.2区域内的rs36605位点多态性与非吸烟者肺癌的易感性有关,尚不能得到MTMR3基因多态性与非吸烟者肺癌的易感性有关。
[Abstract]:Objective the MTMR3(myotubularin related protein 3 gene located on chromosome 22q12.2 is a gene that regulates the expression of muscle vascular bundle protein. The overexpression of MTMR3 gene is related to tumorigenesis and other diseases. The purpose of this study is to explore the expression of quantitative character expression Quati tatire Trait Locie QTL (QTL). The association between MTMR3 gene polymorphism and lung cancer susceptibility in non-smokers. Methods Linkage disequilibrium and eQTL analysis were carried out in 22q12.2 region of lung cancer susceptibility, and pathogenicity sites were screened for gene expression regulation and gene regulation was predicted. A case-control study was used in this study. The cases included 96 cases from Liaoning Provincial Oncology Hospital 2013-03-05-2014-12-30, 92 cases from the first affiliated Hospital of China Medical University, 90 cases from the 4th affiliated Hospital of China Medical University, 95 cases from the General Hospital of Shenyang military region) and 88 cases from Shenyang 202 Hospital of the people's Liberation Army. There were 461 patients with primary lung cancer (case group) and 472 healthy controls (control group) in the third grade A hospital. The rs36605 locus was genotyped by TaqMan genotyping technique. T test was used to compare age in the case group. The difference of distribution between the control group and the control group, 蠂 2 test was used to compare the distribution of sex, genotypes and environmental exposure factors between the case group and the control group. The OR value and its 95% confidence interval were calculated by Logistic regression. Results eQTL analysis showed that the rs36605 locus was a cis QTLL rs36605 locus of MTMR3 gene, which might be related to the expression of MTMR3 gene. There was no significant correlation between the risk of lung cancer and the risk of lung cancer. There was also no significant correlation between the overt model and the invisible model ORA (0.645.895 CI = 0.645.856 P0.238). The results showed that cooking oil smoke exposure increased the risk of lung cancer, and was not found in the dominant model (0.631.15P0.291) or in the invisible model (0.645.895 CI = 0.645.86P0.238), the results showed that cooking oil fume exposure increased the risk of lung cancer. The CI was 1.06 ~ 2.45% P0.025, but there was no interaction between cooking oil fume exposure and polymorphism of rs36605 locus. Conclusion the polymorphism of rs36605 locus in the 22q12.2 region was not associated with the susceptibility of non-smokers to lung cancer. No MTMR3 gene polymorphism has been found to be associated with the susceptibility of non-smokers to lung cancer.
【作者单位】： 中国医科大学公共卫生学院流行病学教研室;
【基金】：中华医学会资助项目(CMB00726)
【分类号】：R734.2
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本文编号：1606421