长期饲喂不同蛋白质水平饲粮对猪脂肪代谢相关基因表达的影响

发布时间：2018-03-15 02:38

本文选题：饲粮　切入点：蛋白质　出处：《动物营养学报》2017年10期 　论文类型：期刊论文

【摘要】：本试验旨在研究长期饲喂不同蛋白质水平饲粮对猪脂肪代谢相关基因表达的影响。试验选用18头三元(杜×长×大)杂交28日龄断奶仔猪,随机分为3组,每组6个重复,每个重复1头。对照组[高粗蛋白质(HCP)组]采用符合NRC(2012)推荐营养需要的饲粮,试验组是根据NRC(2012)标准,在添加赖氨酸(Lys)、蛋氨酸(Met)、苏氨酸(Thr)、色氨酸(Try)4种必需氨基酸基础上,将饲粮蛋白质水平分别降低3%[中粗蛋白质(MCP)组]和6%[低粗蛋白质(LCP)组]。试验期125 d。结果表明:1)在肝脏中,与HCP组相比,LCP组显著降低脂肪酸合成相关基因乙酰辅酶A羧化酶(ACC)、脂肪酸合成酶(FAS)、苹果酸酶1(ME1)及锚蛋白1(ANK1)的基因表达量(P0.05);同时显著提高脂肪酸转运相关基因过氧化物酶体增殖物激活受体γ(PPARγ)及脂肪酸结合蛋白(FABP)的基因表达量(P0.05);而MCP组与HCP组相比,脂肪酸合成、转运相关基因表达量均无显著性差异(P0.05);与HCP组相比,MCP及LCP组均显著降低脂肪酸氧化分解相关基因、过氧化物酶体增殖物激活受体α(PPARα)、肉毒碱棕榈酰转移酶(CPT)的基因表达量(P0.05)。2)在背最长肌(LDM)中,MCP组中脂肪酸合成相关基因胆固醇调节元件结合蛋白(SREBP)、FAS、硬脂酰辅酶A去饱和酶(SCD)的基因表达量显著高于其他2组(P0.05);ACC及FABP在LCP组的基因表达量显著低于其他2组(P0.05);CPT基因表达量及LDM的肌内脂肪(IM F)含量在LCP及M CP组显著低于HCP组(P0.05)。3)肝脏和LDM中,各组甘油三酯脂酶(ATG L)及二烯酰辅酶A还原酶(DECR)基因表达量均无显著性差异(P0.05)。由此可见,在NRC(2012)基础上,适当的降低饲粮蛋白质水平(3%)可促进LDM中脂肪酸合成、转运相关基因的表达,但对肝脏中脂肪酸合成相关基因的表达无显著影响;适当的降低饲粮蛋白质水平(3%)可降低LDM及肝脏中脂肪酸氧化分解相关基因的表达,但并不增加LDM肌内脂肪含量。
[Abstract]:The purpose of this study was to study the effect of long-term feeding on the expression of genes related to fat metabolism in pigs fed with different protein levels. Eighteen piglets were randomly divided into 3 groups with 6 replicates in each group. The control group [high crude protein HCP] group was fed a diet that met the nutritional requirements recommended by NRCf2012. According to NRCf2012), the experimental group was supplemented with four essential amino acids, Lysl, methionine, threonine tachycardia and tryptophan, according to the NRCf2012) standard. Dietary protein levels were reduced by 3% [medium crude protein group] and 6% [low crude protein LCPgroup]. Compared with HCP group, the gene expression of acetyl-coenzyme A carboxylase, fatty acid synthase (FASA), malate enzyme (1nm-1) and anchor protein 1 (ANK1) were significantly decreased in HCP group, and the peroxia gene related to fatty acid transportation-related gene was significantly increased. The gene expression of PPAR 纬) and fatty acid binding protein (Fab) in the chemosomal proliferator activated receptor (PPAR 纬) and fatty acid binding protein (Fab) were P0.05, while in the MCP group, they were compared with those in the HCP group. There was no significant difference in the expression of fatty acid biosynthesis and transport-related genes between MCP and LCP groups, and there was no significant difference between MCP and LCP groups. Peroxisome proliferator activated receptor 伪 -PPAR 伪, carnitine palmitoyltransferase (CPT) gene expression level: P0.05 (2) in LDMgroup of longissimus dorsi (LDM), the fatty acid synthase associated gene cholesterol regulatory element binding protein, SREBPNK FASS, stearyl coenzyme A (stearyl coenzyme A) was saturated in the MCP group. The expression of LCP and FABP in LCP group was significantly lower than that in other two groups. The expression of LDM was significantly lower in LCP and MCP groups than that in HCP group (P 0.05. 3), and in LCP and MCP group, it was significantly lower than that in HCP group (P 0.05. 3), and the expression of LDM was significantly lower in LCP group than that in other two groups (P 0.05. 3), and the expression of LDM was significantly lower in LCP and MCP groups than that in HCP group (P 0. 05. 3). There was no significant difference in the expression of triglyceride lipase (ATG) and diallycoenzyme A reductase (DECR) gene in each group. It can be seen that, on the basis of NRCf2012), the protein level of dietary protein can be reduced properly) to promote the synthesis of fatty acids in LDM. The expression of transporter related genes had no significant effect on the expression of fatty acid biosynthesis related genes in liver, and a proper reduction of dietary protein level could decrease the expression of LDM and fatty acid oxidative decomposition related genes in liver. But it did not increase the intramuscular fat content of LDM.
【作者单位】：广东省农业科学院动物科学研究所畜禽育种国家重点实验室农业部华南动物营养与饲料重点实验室广东省动物育种与营养公共实验室广东省畜禽育种与营养研究重点实验室;
【基金】：国家973计划(2013CB127301,04) 广东省现代农业产业技术体系(2016LM1080) 广东省国际科技合作计划项目(2014A050503049) 国家现代农业产业体系(CAR-36) 省级现代农业(畜禽健康养殖)产业技术研发中心建设项目
【分类号】：S828.5

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