高产与低产蛋鸡卵巢和卵泡及相关基因的表达差异

发布时间：2018-03-15 05:37

本文选题：蛋鸡　切入点：卵泡组织形态　出处：《南京农业大学学报》2017年05期 　论文类型：期刊论文

【摘要】：[目的]以东乡绿壳蛋鸡为研究对象,从形态学和分子水平上探究高低产蛋鸡卵巢和卵泡的差异。[方法]选择高产组、低产组东乡绿壳蛋鸡各10只,采集静脉血,测定相关激素含量;屠宰采集卵巢组织,制作组织切片,观察卵巢形态结构;提取卵巢组织RNA,分别测定高产与低产蛋鸡卵巢中的细胞增殖凋亡基因、生殖调控相关转录因子基因、类固醇激素合成关键酶基因和相关激素受体基因的表达量。[结果]高产与低产蛋鸡卵巢和卵泡在形态上有明显差别:高产蛋鸡卵巢体积大,卵泡丰富,细胞排列紧密;低产蛋鸡卵巢、卵泡发育不良,细胞松散。高产蛋鸡血清中促卵泡激素(FSH)、孕酮和雌二醇水平均显著高于低产蛋鸡(P0.05)。实时定量PCR检测结果显示:在高产蛋鸡中翼状螺旋/叉头转录因子2(FOXL2)、转录因子GATA家族4基因(GATA4)和B淋巴细胞瘤2基因(Bcl2)表达显著上调(P0.05),且在高产蛋鸡卵巢中激活素受体1(ACVR1)和增殖细胞核抗原基因(PCNA)表达量极显著高于低产蛋鸡(P0.01),但细胞色素P450家族11A1基因(CYP11A1)表达量显著低于低产组(P0.05)。[结论]血清中生殖激素含量以及FOXL2、GATA4、Bcl2、ACVR1、PCNA和CYP11A1基因表达的差异是影响鸡产蛋性能高低的重要因素。
[Abstract]:[objective] to study the difference of ovary and follicle between high and low laying hens on morphology and molecular level. [methods] High yield group and low yield group 10 Dongxiang green shell laying hens were selected to collect venous blood. The contents of related hormones were measured, the ovarian tissues were slaughtered and collected, tissue sections were made, ovarian morphology was observed, ovarian tissue RNAs were extracted, and the proliferative and apoptotic genes in the ovary of high-yield and low-laying hens were determined, respectively. The expression of genes related to transcription factor, steroid hormone synthesis key enzyme and related hormone receptor genes in reproductive regulation was significantly different from that in the ovary and follicle of high and low laying hens: the ovarian size of high yield laying hens was large. The follicles are rich in follicles, and the cells are closely arranged. The levels of follicle stimulating hormone (FSH), progesterone and estradiol in the serum of high yield laying hens were significantly higher than those of low laying hens (P0.05). The results of real-time quantitative PCR analysis showed that the winglike helix / fork head transcription factor 2FOXL2 and transcription factor were found in high yield laying hens. The expression of activin receptor 1 (ACVR1) and proliferating cell nuclear antigen (PCNA) gene in ovary of GATA family 4) and B lymphocytoma 2 gene (Bcl2) were significantly higher than those of low laying hens, but cytochrome P450 was significantly higher than that of low laying hens (P 0.01), and the expression of activin receptor 1 (ACVR1) and proliferating cell nuclear antigen gene (PCNA) were significantly higher in the ovary of high-yielding laying hens. The expression of CYP11A1 in family 11A1 gene was significantly lower than that in low productivity group (P 0.05). [conclusion] the difference of serum reproductive hormone level and the expression of FOXL2GATA4Bcl2ACVR1 gene are the important factors that influence the laying performance of chicken.
【作者单位】：南京农业大学动物科技学院;
【基金】：江苏省农业科技自主创新资金项目[CX(13)2038] 江苏省自然科学基金项目(BK20151433) 江苏省农业“三新工程”项目(SXGC[2016]316)
【分类号】：S831

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