海南粗榧PDS基因克隆与茉莉酸甲酯诱导表达分析

发布时间：2018-03-16 07:43

本文选题：海南粗榧　切入点：PDS基因　出处：《分子植物育种》2017年01期 　论文类型：期刊论文

【摘要】：八氢番茄红素脱氢酶(PDS)基因是类胡萝卜素生物合成途径中关键基因,它参与叶绿体光保护、脱落酸合成等多种生物代谢,同时也是VIGS技术中常用的指示基因。本研究利用同源克隆技术克隆并分析了海南粗榧八氢番茄红素脱氢酶(PDS)基因,结果表明:海南粗榧PDS基因,c DNA全长1 758 bp,命名为Cm PDS(Gen Bank登录号:KX766035),其编码585个氨基酸的开放阅读框。预测该基因编码蛋白质分子量为64.99 k D,其等电位为7.11,是理化性质稳定的亲水蛋白。经过氨基酸序列比对,发现海南粗榧中的PDS和红掌、中国水仙的PDS同源性最高,为85%;与其他作物的PDS同源性也都在70%以上,并且包含一个明显的保守结构域。此外,通过施加茉莉酸甲酯来模拟逆境信号,发现Cm PDS基因表达量呈现了先快速下降,然后逐步恢复到正常表达水平的趋势。暗示茉莉酸信号启动的抗性机制会抑制植物的光保护机制。同时本研究也为利用VIGS技术鉴定和挖掘海南粗榧抗癌生物碱合成酶相关基因奠定基础。
[Abstract]:Octahydrolycopene dehydrogenase (PDS) gene is a key gene in carotenoid biosynthesis pathway. It is involved in chloroplast photoprotection and abscisic acid biosynthesis. It is also a commonly used indicator gene in VIGS technology. In this study, we cloned and analyzed the VIGS gene by homologous cloning technique. The results show that the full length of c DNA of PDS gene of Torreya Hainan is 1 758 BP, named cm PDS(Gen Bank accession number: KX766035, which encodes an open reading frame of 585 amino acids. It is predicted that the molecular weight of protein encoded by this gene is 64.99 KD, and its equipotential is 7.11, which is physicochemical. A stable hydrophilic protein. After amino acid sequence alignment, It was found that the homology of PDS in Chinese narcissus was 85.The homology of PDS with other crops was more than 70%, and it contained an obvious conserved domain. By applying methyl jasmonate to simulate the stress signal, it was found that the expression of cm PDS gene decreased rapidly first. The results suggest that the resistance mechanism initiated by jasmonic acid signal will inhibit the photoprotection mechanism of plants. This study also aims to identify and excavate anticancer alkaloids in Torreya hainanensis by VIGS technique. Enzyme-related genes lay the foundation.
【作者单位】：中国热带农业科学院热带作物品种资源研究所农业部华南作物基因资源与种质创制重点开放实验室;海南大学园艺园林学院;
【基金】：国家自然科学基金面上项目(31570326)资助
【分类号】：S791;Q943.2

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