两种水稻短穗突变体的形态特征、遗传分析和基因定位

发布时间：2018-03-16 11:10

本文选题：水稻　切入点：穗长　出处：《基因组学与应用生物学》2016年05期 　论文类型：期刊论文

【摘要】：通过辐射诱变籼稻品种明恢86,获得两种短穗突变体,分别命名为rpl1(reduced panicle length 1)和rpl2(reduced panicle length 2)。两突变体都表现为穗变短、枝梗数和穗粒数减少、枝梗缩短,但其再生植株的穗长有所恢复,与野生型的差异明显减少。等位性测验表明,rpl1和rpl2是等位突变。利用M4分离群体和基于高通量测序的分离体混合分析(BSA-seq)方法,发现rpl2中LOC_Os11g12740基因的剪接位点发生了单碱基置换突变。进一步对rpl1测序分析,发现该基因完全缺失。这说明rpl1和rpl2都是LOC_Os11g12740突变引起的,由此证实它就是目标基因。该基因与已报道的SP1是同一个基因,因此rpl1和rpl2是SP1的新等位突变,但二者的表型与已报道的3种短穗突变体并不完全相同,说明不同突变和遗传背景会影响基因功能的表现。本研究进一步验证了SP1的功能,并为深入研究其分子作用机理提供新材料。
[Abstract]:Two short spike mutants, named rpl1(reduced panicle length 1) and rpl2(reduced panicle length 2, were obtained by radiation-induced transformation of indica rice variety Minghui 86. The two mutants were characterized by shorter panicles, fewer branches and grains and shorter branches. However, the panicle length of regenerated plants recovered, and the difference with wild type was significantly reduced. Allelic test showed that rpl1 and rpl2 were allelic mutations. BSA-seq method was used to isolate M4 population and isolate BSA-seq based on high-throughput sequencing. A single base substitution mutation was found in the splicing site of the LOC_Os11g12740 gene in rpl2. Further analysis of rpl1 sequencing showed that the gene was completely missing, which indicated that both rpl1 and rpl2 were caused by LOC_Os11g12740 mutation. Therefore, rpl1 and rpl2 are new alleles of SP1, but their phenotypes are not identical with the three reported short spike mutants. This study further verifies the function of SP1 and provides a new material for further study of its molecular mechanism.
【作者单位】：作物遗传育种与综合利用教育部重点实验室福建农林大学;福建省作物设计育种重点实验室福建农林大学;
【基金】：福建省自然科学基金项目(2014J01093)资助
【分类号】：S511

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