龙眼多聚半乳糖醛酸酶基因DlPG1表达模式与幼果脱落关系研究

发布时间：2018-03-16 13:12

  本文选题：龙眼　切入点：多聚半乳糖醛酸酶基因　出处：《中国南方果树》2017年03期 　论文类型：期刊论文

【摘要】：从龙眼中分离获得1个多聚半乳糖醛酸酶(PG)基因,命名为DlPG1。DlPG1基因与多种物种PG基因氨基酸序列有较高的同源性,其中与同属于无患子科的荔枝同源性最高,为95%,且与荔枝的亲缘关系最近。DlPG1基因包含PG基因特有4个保守区中的3个,DlPG1属于E进化支PG基因家族成员。环剥摘叶处理可以显著促进龙眼幼果脱落,对照累积落果率为6.12%,处理为89.85%,相对落果率高峰出现在处理后第5天。处理的离区DlPG1基因表达量始终高于对照,且表达量高峰出现在处理后第4天。基因表达量的高峰早于相对落果率高峰出现,据此推测,DlPG1基因可能是调控龙眼幼果脱落的关键基因。截至目前,DlPG1基因是首例在龙眼中发现的属于E进化支的可能与器官脱落相关的PG基因。
[Abstract]:A polygalacturonidase (DlPG1.DlPG1) gene was isolated from longan and named as DlPG1.DlPG1 gene, which has high homology with PG gene of many species, and has the highest homology with Litchi, which belongs to the same family of Litchi. DlPG1 gene is a member of PG gene family of E evolutionary branch, and the treatment of girdling leaves can significantly promote the peeling of longan young fruit, and DlPG1 gene contains three of the four conserved regions of PG gene endemic to Litchi chinensis, which belong to the PG gene family of E evolutionary branch. The cumulative fruit drop rate of the control was 6.12 and that of the treatment was 89.85. The peak of the relative fruit drop rate appeared on the 5th day after treatment. The expression of DlPG1 gene in the isolated region of the treatment was always higher than that of the control. The peak of expression appeared on the 4th day after treatment, and the peak of gene expression was earlier than the peak of relative fruit drop rate. It is suggested that DlPG1 gene may be the key gene to regulate the exfoliation of young longan fruit. So far, DlPG1 gene is the first PG gene found in longan, which belongs to the E evolutionary branch and may be related to organ exfoliation.
【作者单位】： 岭南师范学院基础教育学院;农业部热带果树生物学重点实验室/中国热带农业科学院南亚热带作物研究所;
【基金】：中央级科研院所基本业务费(1630062015004) 广东省湛江市非资助科技攻关计划项目(2016B01106) 岭南师范学院青年项目(QL1514)资助
【分类号】：S667.2
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本文编号：1620066