蒙古马主要性格性状与其候选基因多态性相关性分析

发布时间：2018-03-17 18:23

本文选题：蒙古马　切入点：性格行为　出处：《农业生物技术学报》2017年03期 　论文类型：期刊论文

【摘要】：马(Equus caballus)的性格行为直接决定其是否或如何被调教训练,蒙古马是受外血影响较小的自然品种,因此,蒙古马是研究马性格行为相关多态位点的最适合的品种。本研究旨在识别脑源性神经营养因子基因(brain derived neurotrophic factor,BDNF)、多巴胺-β-羟化酶基因(dopamine beta-hydroxylase,DBH)、5-羟色胺转运体基因(solute carrier family 6 member 4 gene,SLC6A4)和儿茶酚-O-甲基转移酶基因(catechol-O-methyltransferase,COMT)片段的多态性,并分析其与蒙古马性格行为的相关性。选用饲养环境和受调教均相同的60匹蒙古马,编号和颈静脉采血。以实地问卷调查的方法,对能反应马匹性格行为的28个表型性状,进行统计学描述。采用单链构象多态性检测(single strand conformational polymorphism,SSCP)和测序的方法,分析4个性格候选基因的多态性;并进行基因分型,分析其与蒙古马性格性状的相关性。结果表明,蒙古马BDNF基因的222 bp(C→T)和261 bp(G→C)位点均有一个突变,且与咬人行为显著相关;DBH基因的第758 bp处有一个(G→A)突变,与对其他马友好和忍耐力行为显著相关,且与理解力、过河、游泳和沙浴等行为相关极显著;SLC6A4基因的第1 362 bp处有一个(T→C)突变,与神经质、恐慌性、对马具的反应和胆怯性等行为相关,与独立性行为相关极显著;COMT基因的第217 bp处有一个(G→A)突变,显著影响蒙古马的竞争性行为。这些数据说明,BDNF、DBH、SLC6A4和COMT基因与蒙古马的性格行为性状相关,而且SLC6A4基因的多态位点在蒙古马以后的进化过程中会稳定遗传。本研究为建立蒙古马性格行为性状的分子标记辅助选择提供了理论依据。
[Abstract]:The personality and behavior of Equus caballus) directly determine whether or how they are trained. Mongolian horse is a natural breed with less influence from external blood, so, Mongolian horse is the most suitable variety to study the polymorphism of personality and behavior in horse. The aim of this study was to identify brain-derived neurotrophic factor gene brain derived neurotrophic factor BDNFN, dopamine-beta-hydroxylase gene, beta-hydroxylase gene, and 5-hydroxytryptamine transporter gene, absolute carrier family 6, to identify brain-derived neurotrophic factor gene brain-derived neurotrophic factor gene brain derived neurotrophic factor BDNF and dopamine-beta-hydroxylase gene. The polymorphism of member 4 gene SLC6A4) and catechol-O-methyltransferase gene (catechol-O-methyltransferase) fragment, The correlation between them and Mongolian horse's character and behavior was analyzed. 60 Mongolian horses with the same feeding environment and training were selected, the blood was collected by serial number and jugular vein. The 28 phenotypic traits that could reflect the horse's character and behavior were investigated by questionnaire survey in the field. The single strand conformational polymorphism (SSCP) and sequencing were used to analyze the polymorphism of four personality candidate genes, and genotyping was carried out to analyze the correlation between single strand conformational polymorphism and Mongolian horse character traits. 222 bp(C of Mongolian Horse BDNF Gene. 鈫扵) and 261 bp(G. 鈫扵here was a mutation at the C) locus, and there was a G at the 758bp of the 758bp gene associated with biting behavior. 鈫扐) mutation, which is significantly associated with friendly and endurance behaviour towards other horses, as well as with behavior such as comprehension, river crossing, swimming and sand bathing, has a T at the 1,362bp site of the SLC6A4 gene. 鈫扖) mutation, associated with neuroticism, panic, response to harness and cowardice, and significant correlation with independent behavior with a G at the 217th BP of COMT gene. 鈫扵hese data indicate that the BDNF DBHG SLC6A4 and COMT genes are associated with the personality and behavior traits of Mongolian horses. The polymorphic loci of SLC6A4 gene are stable in the evolution of Mongolian horses. This study provides a theoretical basis for the establishment of molecular marker-assisted selection of Mongolian horse personality and behavior traits.
【作者单位】：内蒙古农业大学动物科学学院/内蒙古自治区蒙古马遗传资源保护及马产业工程实验室;内蒙古疾病预防控制中心;
【基金】：国家自然科学基金项目(No.31360538和No.31472070) 内蒙古自治区科技厅重点实验室建设项目(No.20130902)
【分类号】：S821

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