水稻Bph14基因生理功能初探

发布时间：2018-03-17 18:36

本文选题：Bph基因　切入点：乙烯合成　出处：《生物技术通报》2017年12期 　论文类型：期刊论文

【摘要】：Bph14基因是第一个被克隆的水稻抗虫基因,但鲜有Bph14基因对水稻生理方面影响的报道。研究该基因对水稻生理方面的影响有助于全面理解Bph14基因对褐飞虱的抗性机制。将Bph14基因转入水稻栽培品种中,对携带有外源基因的转基因株系通过荧光定量PCR检测乙烯合成和非生物胁迫相关基因的表达,另外,分别用5μmol/L ABA和250 mmol/L Na Cl处理转基因水稻株系和相应受体材料,结果表明,与乙烯合成相关的基因Os ACO2,Os ACS2,Os ACS6,O1g P5CS和O5g P5CS的表达量均较转基因受体m5274发生了变化,与干旱、耐盐等非生物胁迫相关的基因Cat A、Cat B、Cat C、RAB16A、LEA3、LIP9、Sal T、Adh I等也都不同于在受体m5274中的表达。同时Bph14基因的导入也提高了水稻对ABA的敏感性和对盐胁迫的耐受性,说明Bph14基因在水稻中可能参与调控乙烯合成以及非生物胁迫应答的功能基因的表达。
[Abstract]:The Bph14 gene is the first rice insect-resistant gene to be cloned. However, there are few reports on the effects of Bph14 gene on rice physiology. It is helpful to understand the resistance mechanism of Bph14 gene to brown planthopper, and to transfer Bph14 gene into rice cultivar. The expression of ethylene biosynthesis and abiotic stress related genes was detected by fluorescence quantitative PCR in transgenic lines carrying exogenous genes. In addition, transgenic rice lines and corresponding receptor materials were treated with 5 渭 mol/L ABA and 250 mmol/L NaCl, respectively. The expression of the genes related to ethylene synthesis, Os ACO2, Os ACS2, Os ACS6, O1g P5CS and O5g P5CS, changed compared with the transgenic receptor m5274. The genes related to abiotic stress, such as salt tolerance, such as Cat Cat bat Cat Cat, RAB16A, LEA3, LIP9, Sal Ta-Adh I, were also different from those expressed in receptor m5274. The introduction of Bph14 gene also enhanced the sensitivity of rice to ABA and its tolerance to salt stress. These results suggest that Bph14 gene may be involved in the regulation of ethylene synthesis and the expression of functional genes in abiotic stress response in rice.
【作者单位】：湖北省农业科学院粮食作物研究所粮食作物种质创新与遗传改良湖北省重点实验室;主要粮食作物产业化湖北省协同创新中心(长江大学);
【基金】：国家转基因重大专项(2014ZX0800101B) 国家科技支撑计划(2015BAD01B01)
【分类号】：S511

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