我国小反刍兽疫病毒H基因序列分析

发布时间：2018-03-17 20:12

  本文选题：小反刍兽疫　切入点：H基因　出处：《江苏农业科学》2017年07期 　论文类型：期刊论文

【摘要】：为了分析我国小反刍兽疫疫情的特点,从GenBank下载我国和其他国家小反刍兽疫病毒(PPRV)毒株H基因全序列,应用分子生物学软件DNAStar进行序列分析。结果显示,始于2007年的我国首次PPR疫情中,我国西藏地区PPRV各毒株间H基因核苷酸同源性介于99.6%~100.0%之间,我国西藏与其他国家PPRV毒株核苷酸同源性介于86.7%~98.3%之间;我国西藏地区PPRV毒株间氨基酸序列同源性介于99.7%~100.0%之间,我国西藏与其他国家PPRV毒株氨基酸序列同源性介于89.3%~98.4%之间。始于2013年年底的我国又一次PPR疫情中,我国PPRV毒株间核苷酸同源性介于99.7%~99.8%之间,我国与其他国家PPRV毒株核苷酸同源性介于87.2%~97.5%之间;我国PPRV毒株间氨基酸序列同源性介于99.2%~99.8%之间,我国与其他国家PPRV毒株氨基酸序列同源性介于89.0%~98.4%之间。H基因进化树分析结果显示,我国的9株PPRV划分为基因Ⅳ系。我国PPRV毒株与印度毒株及土耳其毒株同源性最高,可能由这2个国家传入我国。
[Abstract]:In order to analyze the characteristics of the epidemic situation of small ruminant in China, the whole H gene sequence was downloaded from GenBank in our country and other countries, and the sequence analysis was carried out by using the molecular biology software DNAStar. In the first PPR epidemic in China, which began in 2007, the nucleotide homology of H gene among PPRV strains in Tibet was between 99.6% and 100.0%, and the nucleotide homology of PPRV strains in Tibet and other countries was between 86.7% and 98.3%. The homology of amino acid sequence among PPRV strains in Tibet region was between 99.70.0% and 98.4% between Tibet and other countries. The homology of amino acid sequence of PPRV virus strains in Tibet and other countries was between 89.3% and 98.4%. The sequence homology of amino acid sequence of PPRV virus strains in China began at the end of 2013 in another PPR epidemic in China. The nucleotide homology of PPRV strains in China was between 99.7% and 99.8%, the nucleotide homology of PPRV strains in China and other countries was between 87.2% and 97.5%, and the amino acid sequence homology of PPRV strains in China was between 99.2% and 99.8%. The amino acid sequence homology of PPRV strains in China and other countries was between 89.0% and 98.4%. The result of phylogenetic tree analysis showed that 9 PPRV strains in China were classified into gene 鈪，

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