LPL基因多态性与代谢综合征“痰证”病位、病性兼杂规律的相关性研究

发布时间：2018-03-18 17:42

本文选题：代谢综合征　切入点：痰证　出处：《中华中医药杂志》2017年07期 　论文类型：期刊论文

【摘要】：目的:探讨LPL基因多态性位点rs10503669、rs17411031与代谢综合征(MS)痰证病位、病性兼杂规律的相关性。方法:收集MS痰证患者183例,MS非痰证患者93例,健康人196名,采用证素辨证法收集分析各证素分布情况;采用SNPscanTM多重SNP分型技术方法,检测LPL单核苷酸多态性(SNPs)位点rs10503669、rs17411031的基因型频率、等位基因频率及其与痰证病位、病性兼杂规律相关性。结果:(1)LPL多态性位点rs10503669的3种基因型频率在3组之间差异有统计学意义(P0.05);痰证组风险基因型纯合子AA与杂合子CA比例升高,与非痰证组比较,差异有统计学意义(P0.05);痰证组风险等位基因A频率高于非痰证组,差异有统计学意义(P0.05)。(2)LPL多态性位点rs17411031的3种基因型频率在3组之间差异有统计学意义(P0.05);非痰证组风险基因型纯合子CC、GG比例升高,与正常组比较,差异有统计学意义(P0.05);非痰证组风险等位基因C频率高于正常组和痰证组(P0.05)。(3)LPL多态性位点rs10503669痰证病性证素湿在风险基因型AA、CC的分布比例上升高(P0.05)。(4)LPL多态性位点rs17411031痰证病位证素肾、病性证素湿在风险基因型CC分布比例上均升高(P0.05)。结论:LPL基因多态性位点rs10503669与MS痰证的形成有关,同时可能与MS痰证兼杂病性湿的形成相关;多态性位点rs17411031与痰证的形成无关,但该位点可能与MS痰证的病位肾、兼杂病性湿有关。
[Abstract]:Objective: to investigate the relationship between the polymorphism of LPL gene rs10503669 / rs17411031 and the status of sputum syndrome, disease and heterogeneity in patients with metabolic syndrome. Methods: 183 patients with MS phlegm syndrome were collected from 93 patients with MS non-phlegm syndrome and 196 healthy persons. The distribution of syndromes was collected and analyzed by syndrome differentiation, and the genotype frequency and allele frequency of LPL single nucleotide polymorphism (LPL) locus rs10503669 / rs17411031 were detected by SNPscanTM multiplex SNP typing technique. Results the frequency of three genotypes of rs10503669 was significantly different among the three groups, and the proportion of homozygous AA and heterozygous CA in risk genotype of phlegm syndrome group was higher than that of non-sputum syndrome group. The risk allele A frequency of phlegm syndrome group was higher than that of non-phlegm syndrome group. There were significant differences in the frequency of three genotypes of rs17411031 among the three groups, and the proportion of homozygotes in the non-phlegm syndrome group was higher than that in the normal group. The risk allele C frequency of non-phlegm syndrome group was higher than that of normal group and phlegm syndrome group. The distribution of rs10503669 phlegm syndrome syndrome syndrome factor dampness in risk genotype AACC was higher than that of normal group and phlegm syndrome group. The distribution ratio of phlegm syndromes syndrome syndrome factor dampness in risk genotype AACC was higher than that in control group and phlegm syndrome group, and the distribution ratio of rs17411031 sputum syndromes was higher than that of normal group and phlegm syndrome group. Conclusion the polymorphic loci rs10503669 of the 1: LPL gene is related to the formation of MS phlegm syndrome, and may be related to the formation of MS phlegm syndrome, while the polymorphic rs17411031 locus is not related to the formation of sputum syndrome. However, this locus may be related to the diseased kidney of MS phlegm syndrome and complicated dampness.
【作者单位】：福建中医药大学;福建中医药大学附属晋江中医院;福建省中医健康状态辨识重点实验室;
【基金】：国家自然科学基金项目(No.81273666) 福建省中医药科研项目(No.wztn201309) 国家中医药管理局项目中医药防治重大疑难疾病(代谢综合征)临床服务能力建设~~
【分类号】：R259

【参考文献】