芬顿法深度处理生物处理排水中的四环素抗性基因

发布时间：2018-03-18 17:48

本文选题：四环素抗性基因　切入点：芬顿氧化　出处：《中国环境科学》2017年09期 　论文类型：期刊论文

【摘要】：以四环素抗性基因tet(L)、tet(E)为研究对象,考察了芬顿氧化技术对四环素抗性基因废水深度处理的效果.采用定量PCR与DGGE技术检测了芬顿法处理前后生物处理排水中的四环素抗性基因的丰度与废水中菌群丰度的变化.结果显示,在最佳反应条件:反应时间10min,p H值为5,[H_2O_2]/[Fe~(2+)](物质量的比)=8且H2O2的浓度达到0.2mol/L时,16Sr RNA及四环素抗性基因(tet genes)的去除效果最好.同时分析芬顿氧化前后的DGGE图谱可知,细菌菌群间的丰度有了明显的变化.相关性分析可知,各目标基因与单因素间、各目标基因之间,各样品中目标基因含量的变化与DGGE条带变化之间都没有明显相关性.结果表明,芬顿试剂可能不仅仅是通过削减单位水体中抗性菌的浓度来降低抗性基因浓度,还通过其他的方式来去除单位水体中的抗性基因的浓度,这还有待进一步研究证明.
[Abstract]:The tetracycline resistance gene (Tet) was studied. The effect of Fenton oxidation on the advanced treatment of tetracycline resistance gene wastewater was investigated. Quantitative PCR and DGGE techniques were used to detect the abundance of tetracycline resistance gene and the flora in wastewater before and after treatment by Fenton method. Changes in abundance. The results show that. Under the optimum reaction conditions: the reaction time is 10 min, the pH value is 5, [H _ S _ 2O _ 2] / [Fe~(2] (the mass ratio is 8 and the concentration of H _ 2O _ 2 is 0.2mol / L), the removal efficiency is best when the concentration of H _ 2O _ 2 is 0.2mol / L. the DGGE profiles before and after Fenton oxidation are also analyzed. The abundance of bacterial flora has changed obviously. The correlation analysis shows that the target genes are among the single factor, the target genes and the target genes. There was no significant correlation between the change of target gene content and the change of DGGE band in each sample. The results showed that Fenton reagent might not only reduce the concentration of resistant bacteria in unit water, but also decrease the concentration of resistant gene. There are other ways to remove the concentration of resistance gene in unit water, which needs further study.
【作者单位】：中国环境科学研究院城市水环境科技创新基地;中国地质大学(北京)水资源与环境学院;中国矿业大学(北京)化学与环境工程学院;阿尔伯特大学土木与环境工程系;
【基金】：中央级公益性科研院所基本科研业务专项(2016YSKY-027) 国家环境保护环境微生物利用与安全控制重点实验室开放基金资助(MARC 2012D008)
【分类号】：X703

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