外源水杨酸诱导的部分甜瓜自毒作用相关基因的表达分析

发布时间：2018-03-19 10:18

  本文选题：甜瓜　切入点：自毒作用　出处：《热带作物学报》2017年05期 　论文类型：期刊论文

【摘要】：以甜瓜(Cucumis melo)品种‘新银辉’为材料,采取根灌法,以水杨酸(10μmol/L)处理甜瓜幼苗,通过Real-Time PCR对叶片中部分自毒作用相关基因的表达量进行定量分析,以探究外源水杨酸诱导对甜瓜自毒作用的影响。结果显示,经外源水杨酸诱导后:(1)在催化苯丙烷类代谢途径的3个初始反应的蛋白酶编码基因中,PAL和C4H随处理后时间增加,呈逐渐上调趋势,但PAL比C4H更快响应胁迫,而4CL转录水平没有显著变化;(2)在类黄酮生物合成的3个关键酶的基因中,CHI、F3H均显示出不同程度的下调,CHS于处理后1 d略微上调,然后恢复至处理前水平;(3)两个转录因子基因WD40、R2R3-MYB分别在不同处理时间后显著下调;(4)WD40与CHI的表达相关性较高。据上述结果 ,水杨酸诱导后:(Ⅰ)CHI表达可能与转录因子WD40蛋白相关;(Ⅱ)WD40、R2R3-MYB与PAL、C4H、4CL、CHS间不存在简单、明确的调控关系;(Ⅲ)柚皮苷、表儿茶素、芦丁等甜瓜主要化感物质合成减少。
[Abstract]:The seedling of muskmelon was treated with 10 渭 mol / L salicylate by root irrigation with Cucumis meloides as material. The expression of some autotoxic genes in leaves was quantitatively analyzed by Real-Time PCR. The effects of exogenous salicylic acid on autotoxicity of muskmelon were studied. The results showed that the effects of exogenous salicylic acid on the autotoxicity of muskmelon were investigated. The results showed that the expression of pal and C4H in the three protease coding genes that catalyze the metabolic pathway of phenylpropanes increased with the treatment of exogenous salicylic acid. However, the transcription level of 4CL did not change significantly. The genes of three key enzymes of flavonoid biosynthesis showed different down-regulation of CHII-F3H, which was slightly up-regulated on the 1st day after treatment, but PAL was more responsive to stress than that of C4H, but the transcription level of 4CL was not changed significantly. Two transcription factor genes, WD40, R2R3-MYB, significantly down-regulated the expression of WD40, R2R3-MYB and CHI, respectively. After salicylic acid induction, the expression of WD40 may be related to the transcription factor WD40 protein (鈪，

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