基于线粒体Cyt b基因的虾夷扇贝群体遗传结构分析

发布时间：2018-03-20 09:24

本文选题：虾夷扇贝　切入点：Cyt　出处：《中国水产科学》2017年03期 　论文类型：期刊论文

【摘要】：本研究利用线粒体细胞色素b(Cyt b)基因标记对虾夷扇贝(Patinopecten yessoensis)6个群体(长岛底播增殖群、海洋岛底播增殖群、獐子岛底播增殖群、旅顺自然群、"獐子红"人工选育群以及日本青森陆奥湾群)进行种质状况评估,研究结果表明,6个群体120个个体的Cyt b基因序列共检测到20种单倍型,其中"獐子红"人工选育群单倍型最少,日本群体单倍型最为丰富,两者单倍型多样性指数分别为0.10000和0.88400。分子方差分析(AMOVA)发现,日本群体与中国群体间变异百分比为15.34%,明显高于作为一个基因池时群体间遗传变异水平;就中国组群而言,其83.41%的遗传变异来自于群体内部,组内群体间的变异只占1.52%,说明中国群体个体间的遗传变异远大于群体间的遗传变异。F_(st)分析显示,中国群体与日本群体之间发生了中等水平的遗传分化(0.07455~0.17895,Fst0.05)。以遗传距离矩阵构建群体间分子系统树(UPGMA树),拓扑结构图显示中国5个群体先聚为一支,再与日本群体聚为一支。由此可见,中国养殖区的虾夷扇贝与日本原产地群体间已出现明显的遗传分化,且虾夷扇贝中国群体的遗传多样性处于较低水平。本研究结果可为虾夷扇贝的种质资源保护与可持续开发利用提供理论依据。
[Abstract]:In this study, six populations of Patinopecten yessoensis (Patinopecten yessoensis) were identified by mitochondrial cytochrome B cyt (Cyt) gene marker, including long island bottom seeding multiplication group, ocean island bottom seeding multiplication group and Swertia island bottom seeding multiplication group. The results showed that 20 haplotypes were detected in 120 individuals of 6 populations, and 20 haplotypes were detected in 120 individuals of 6 populations. The population haplotypes were the least, and the haplotypes were the most abundant in Japan. The diversity indices of the two haplotypes were 0.10000 and 0.88400, respectively. The results of molecular analysis of variance (AMOVA) showed that. The percentage of variation between Japanese population and Chinese population was 15.34, which was significantly higher than that between populations as a gene pool, and 83.41% of the genetic variation in Chinese population came from within the population. The variation among populations within groups was only 1.52%, which indicated that the genetic variation among individuals in Chinese population was much larger than that among populations. There was a moderate genetic differentiation between Chinese population and Japanese population. The genetic distance matrix was used to construct the molecular phylogenetic tree of UPGMA. The topological map showed that the five populations of China first converged into one branch. From this, we can see that there has been obvious genetic differentiation between the scallop and the population of Japanese origin in the Chinese culture area. The genetic diversity of scallop scallop populations in China is at a low level. The results of this study can provide a theoretical basis for the conservation of germplasm resources and sustainable development and utilization of scallop scallop.
【作者单位】：上海海洋大学水产与生命学院;中国水产科学研究院黄海水产研究所;青岛海洋科学与技术国家实验室海洋渔业科学与食物产出过程功能实验室;大连海洋大学水产与生命学院;
【基金】：“十二五”农村领域国家科技计划项目(2012BAD18B03) 山东省泰山学者建设工程专项
【分类号】：S917.4

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