中间锦鸡儿CiPP2C8-like基因的克隆及表达分析

发布时间：2018-03-21 02:30

本文选题：中间锦鸡儿　切入点：PPC　出处：《基因组学与应用生物学》2017年09期 　论文类型：期刊论文

【摘要】：蛋白磷酸酶PP2C作为调节因子直接或间接调控逆境胁迫信号途径和生长发育过程。本研究从已构建好的抑制性消减文库(SSH)中获得1条PP2C基因序列,对其进行了克隆。测序表明该基因c DNA长为999 bp,共编码333个氨基酸,推导该蛋白分子量为36.12 k D,等电点为6.71,是一种亲水蛋白。经氨基酸序列比对和进化树分析表明,该基因属于PP2C家族成员,并命名为Ci PP2C8-like。实时荧光定量PCR检测表明中间锦鸡儿Ci PP2C8-like基因表达受盐和脱水胁迫诱导。
[Abstract]:As a regulatory factor, protein phosphatase (PP2C) directly or indirectly regulates stress signaling pathway and growth and development. In this study, a PP2C gene sequence was obtained from the constructed suppression subtractive library. The cDNA was cloned. The result of sequencing showed that the gene was 999bplong, encoding 333amino acids, deduced that the molecular weight of the protein was 36.12kD and the isoelectric point was 6.71.The results of amino acid sequence alignment and phylogenetic tree analysis showed that the protein was hydrophilic. The gene belongs to the PP2C family and is named Ci PP2C8-like. The real-time fluorescence quantitative PCR analysis showed that the expression of ci PP2C8-like gene in Caragana intermedia was induced by salt and dehydration stress.
【作者单位】：内蒙古农业大学生命科学学院;内蒙古生物技术研究院;
【基金】：国家自然科学基金(31760696) 内蒙古自治区科技创新团队(201503004)资助
【分类号】：Q943.2;S793.3

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