广东省部分地区奶牛乳房炎凝固酶阴性葡萄球菌的毒力基因和耐药性分析

发布时间：2018-03-21 09:19

本文选题：乳房炎　切入点：凝固酶阴性葡萄球菌　出处：《中国兽医学报》2017年08期 　论文类型：期刊论文

【摘要】：为了解广东省部分地区奶牛乳房炎凝固酶阴性葡萄球菌(coagulase negative Staphylococci,CNS)的毒力基因的分布情况和耐药现状,进而为奶牛乳房炎的防治提供依据。本试验对广东省部分地区奶牛场110份乳房炎奶样中分离出的40株CNS采用PCR方法检测毒力基因、耐药基因和耐消毒剂基因,K-B试纸片法检测药敏性。结果表明CNS毒力基因检测中检测率最高的为fnbp(17.5%),其次为seb(15%)和tsst(15%),毒力基因型复杂(15种)。耐药性检测中对链霉素、红霉素和青霉素G耐药率较高,对苯唑西林、头孢曲松和头孢唑啉较敏感,多重耐药率高(52.5%)。耐药基因检测率最高的为喹诺酮类基因qnrA/B/C/D(20%),耐药基因链霉素耐药表型主要由aac6-aph2介导,耐消毒剂基因检测率最高的为qacG(25%),耐药基因和耐消毒剂基因的基因组型复杂(14种)。
[Abstract]:In order to understand the distribution of virulence gene and drug resistance of Coagulase negative Staphylococcus staphylococcus in dairy cow mastitis coagulase negative staphylococcus in Guangdong Province. 40 strains of CNS isolated from 110 milk samples from dairy cattle farms in Guangdong Province were tested for virulence genes by PCR method. The results showed that the highest detection rate of CNS virulence gene was fnbpnp1. 17.5%, followed by sebpentine (15) and TSS 1515, the virulence genotypes were complex, 15 strains of streptomycin were detected in CNS virulence gene test. The resistance rate of erythromycin and penicillin G was high, sensitive to oxacillin, ceftriaxone and cefazolin, and the rate of multidrug resistance was 52.5%. The highest detection rate of quinolone gene was QnrAr / B / C / C / C / D 20, and the resistance phenotype of streptomycin resistance gene was mainly mediated by aac6-aph2. The highest detection rate of disinfectant genes was qacGf25m, and 14 species of complex genometries of drug-resistant genes and disinfectant resistant genes were identified.
【作者单位】：华南农业大学兽医学院;广东省兽医临床重大疾病综合防控重点实验室;广东省宠物工程技术研究中心;
【基金】：广东省科技计划资助项目(2015B020203005,2015A020224039)
【分类号】：S858.23

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