假禾谷镰孢侵染小麦后3种植物激素相关基因的差异表达分析

发布时间：2018-03-21 11:35

本文选题：小麦　切入点：假禾谷镰孢　出处：《作物学报》2017年11期 　论文类型：期刊论文

【摘要】：假禾谷镰孢(Fusarium pseudograminearum)是我国近年新发现的小麦病原真菌,本研究目的是揭示植物激素信号传导途径中相关基因表达对假禾谷镰孢侵染的响应。利用假禾谷镰孢野生菌株WZ2-8A侵染小麦品种周麦24,对接种后5 d和15 d样品进行转录组测序,分析差异表达基因,并对候选基因进行q RT-PCR验证。假禾谷镰孢侵染后,小麦幼苗生长受到明显抑制,根长、株高、根重和地上部分鲜重均显著降低。转录组分析结果表明,植物激素信号传导途径中有29个基因差异表达,涉及到生长素、细胞分裂素和脱落酸3种植物激素。在接种后5 d有11个差异表达基因,其中2个上调,9个下调;在接种后15 d共有25个差异表达基因,其中8个上调,17个下调。在生长素信号传导途径中,生长素输入载体AUX1差异表达,影响生长素的极性运输,从而影响小麦根部的细胞伸长。在细胞分裂素信号传导途径中,起正调控作用的B-ARR上调表达,推测其促进细胞分裂素的信号传导,从而抑制细胞分裂,与生长素协同作用,造成小麦的长势减弱。在脱落酸途径中,脱落酸受体PYR/PYL下调表达;起到负调控作用的PP2C相关基因均上调表达。脱落酸使植物对真菌和细菌的抗性起到负调控作用,其信号传导途径与茉莉酸/乙烯途径相互拮抗,脱落酸信号传导途径的阻遏可能会使茉莉酸/乙烯途径信号通路打开。q RT-PCR结果基本能够和转录组测序结果相拟合,说明在假禾谷镰孢侵染胁迫下,脱落酸的信号传导被抑制可能是中抗品种周麦24对假禾谷镰孢产生一定的抗性的生理基础。
[Abstract]:Fusarium pseudograminearum (Fusarium pseudograminearum) is a new pathogen of wheat in China. The purpose of this study was to reveal the response of gene expression related to plant hormone signal transduction pathway to the infection of Fusarium graminearum. The wheat variety Zhoumai 24 was infected by WZ2-8A, and the samples were sequenced 5 and 15 days after inoculation. The differentially expressed genes were analyzed, and the candidate genes were verified by Q RT-PCR. The growth of wheat seedlings was significantly inhibited, root length, plant height, root weight and aboveground fresh weight were significantly decreased after infection by Fusarium graminearum. There were 29 differentially expressed genes in plant hormone signal transduction pathway, involving auxin, cytokinin and abscisic acid. There were 11 differentially expressed genes 5 days after inoculation, 2 of which were up-regulated and 9 were down-regulated. On the 15th day after inoculation, there were 25 differentially expressed genes, of which 8 were up-regulated and 17 down-regulated. In the auxin signaling pathway, auxin input vector AUX1 differentially expressed, which affected the polar transport of auxin. In the cytokinin signal transduction pathway, B-ARR, which plays a positive role in regulating the expression of B-ARR, can promote the signal transduction of cytokinin, thus inhibiting cell division and synergism with auxin. In the abscisic acid pathway, abscisic acid receptor PYR/PYL down-regulated the expression of PP2C related genes. Abscisic acid caused the negative regulation of plant resistance to fungi and bacteria. The inhibition of abscisic acid signal transduction pathway may make the signal pathway of jasmonic acid / ethylene pathway open. Q RT-PCR results can basically match the results of transcriptome sequencing. It was suggested that the inhibition of abscisic acid signal transduction might be the physiological basis of resistance to Fusarium graminearum in the middle resistant cultivar Zhoumai 24 under infection stress of Fusarium graminearum.
【作者单位】：河南农业大学/小麦玉米作物学国家重点实验室/河南粮食作物协同创新中心;
【基金】：国家公益性行业(农业)科技专项经费(201503112)资助~~
【分类号】：S435.12

【相似文献】