不同生物系统中β-内酰胺抗性基因的变化规律

发布时间：2018-03-21 12:16

本文选题：抗性基因　切入点：接种污泥　出处：《环境科学与技术》2017年08期 　论文类型：期刊论文

【摘要】：近年来,抗生素抗性基因作为一种新兴的环境污染物,已经在多种水环境中被检测出。该文研究比较了接种污泥(IS)、传统活性污泥工艺(ASC)、序批式生物膜反应器(SBBR)中抗性基因的变化规律。3种系统均检测出了OXA-1、OXA-2以及OXA-10 3种-内酰胺抗性基因。该文还通过实时定量PCR技术对3种抗性基因进行定量检测。结果表明,传统活性污泥工艺对β-内酰胺抗性基因去除效果更好。增加抗生素的初始浓度可以有效去除ASC及SBBR系统中携带抗性基因的抗性微生物。研究还表明,若停止对3个生物系统中加抗生素,并运行10个反应周期(每个周期为12 h)后,3个系统的抗性基因浓度均有所降低。然而,若使其在低抗生素条件下运行10个周期后,3个生物系统的抗性基因浓度均有所增加。
[Abstract]:In recent years, antibiotic resistance genes as a new environmental pollutant, It has been detected in a variety of water environments. In this paper, the variation of resistance genes in inoculated sludge, traditional activated sludge process and sequencing batch biofilm reactor has been studied. 3 systems have detected three kinds of OXA-1 OXA-2 and OXA-10. -lactam resistance genes. Three resistant genes were quantitatively detected by real-time quantitative PCR. The traditional activated sludge process is more effective in removing 尾 -lactam resistance genes. Increasing the initial concentration of antibiotics can effectively remove the resistant microorganisms carrying resistance genes in ASC and SBBR systems. The concentration of resistant genes in 3 biological systems decreased after the antibiotic treatment was stopped and 10 reaction cycles were performed (12 h each cycle). The concentration of resistant genes in all three biological systems increased after 10 cycles under low antibiotic conditions.
【作者单位】：北京化工大学化学工程学院;北京市环境污染控制与资源化工程研究中心;
【基金】：国家自然科学基金项目(51178022);国家自然科学基金项目(51278022)
【分类号】：X703

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