乌鲁木齐市耐多药结核分枝杆菌基因突变特征分析

发布时间：2018-03-21 21:33

本文选题：分枝杆菌　切入点：非典型性　出处：《中华疾病控制杂志》2017年01期 　论文类型：期刊论文

【摘要】：目的了解耐四种一线抗结核药物结核分枝杆菌的有关基因突变特征。方法对19例耐多药结核病患者和254例全敏结核病患者痰液标本中分离的结核分枝杆菌进行药物敏感性试验,提取其DNA进行PCR扩增,对扩增产物测序并与标准株H37Rv进行比对。结果 19株耐多药结核杆菌和254株全敏结核杆菌均未检测到耐异烟肼基因inhA突变;耐多药组突变率最高的为耐异烟肼kat G基因,突变位点为315;耐利福平的突变基因是rpo B,耐药位点为526和531,531位点突变率高于526位点;耐乙胺丁醇的突变基因是emb B,耐药位点为206;耐链霉素的突变基因是rps L和rrs,耐药位点分别为43和1401,rps L基因的突变率高于rrs基因。结论耐多药结核分枝杆菌耐异烟肼、利福平、乙胺丁醇、链霉素的突变基因分别为kat G、rpo B、emb B、rps L和rrs,这对今后乌鲁木齐市耐多药结核病的快速诊断和控制提供了理论依据。
[Abstract]:Objective to investigate the characteristics of gene mutations in mycobacterium tuberculosis resistant to four first-line anti-tuberculosis drugs. Methods Drug sensitivity tests were performed in sputum samples of 19 patients with multidrug-resistant tuberculosis and 254 patients with hypersensitive tuberculosis. The DNA was extracted for PCR amplification, the amplified products were sequenced and compared with the standard strains H37Rv. Results No inhA mutation of isoniazid resistant gene was detected in 19 strains of multidrug resistant Mycobacterium tuberculosis and 254 strains of allelic Mycobacterium tuberculosis. The mutation rate of isoniazid resistant kat G gene was the highest in multidrug resistance group, and the mutation rate of rifampicin-resistant gene was rpo B, and the mutation rate of resistant site 526 and 531531 was higher than that of isoniazid resistance gene, and the mutation rate of rifampicin-resistant gene was higher than that of isoniazid resistance group. The mutant gene resistant to ethambutanol is emb B with a drug resistance site of 206, and streptomycin resistant mutant gene is rps L and rrs. The mutation rate of resistant locus is 43 and 1401 rps L, respectively, which is higher than that of rrs gene. Conclusion the multidrug resistant Mycobacterium tuberculosis is isoniazid and rifampicin resistant to isoniazid and rifampicin. The mutated genes of ethambutanol and streptomycin are kat Gnrpo Bemb Bnrps L and rrs, respectively, which provides a theoretical basis for the rapid diagnosis and control of MDR-TB in Urumqi.
【作者单位】：新疆医科大学公共卫学院流行病与卫生统计教研室;乌鲁木齐市疾病预防控制中心结核病防治科;新疆军区疾病预防控制中心消杀灭科;
【基金】：新疆维吾尔自治区自然科学基金项目(201442137-20) 乌鲁木齐市卫生局科学技术计划项目(201313)
【分类号】：R52

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